Disaturated-phosphatidylcholine and Surfactant protein-B turnover in human acute lung injury and in control patients

Manuela Simonato, Aldo Baritussio, Carlo Ori, Luca Vedovelli, Sandra Rossi, Lorenza Dalla Massara, Sabina Rizzi, Virgilio P. Carnielli, Paola E. Cogo

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Abstract

Background: Patients with Adult Respiratory Distress Syndrome (ARDS) and Acute Lung Injury (ALI) have low concentrations of disaturated-phosphatidylcholine and surfactant protein-B in bronchoalveolar lavage fluid. No information is available on their turnover. Objectives: To analyze disaturated-phosphatidylcholine and surfactant protein-B turnover in patients with ARDS/ALI and in human adults with normal lungs (controls). Methods: 2H2O as precursor of disaturated-phosphatidylcholine-palmitate and 1 13C-Leucine as precursor of surfactant protein-B were administered intravenously to 12 patients with ARDS/ALI and to 8 controls. Disaturated-phosphatidylcholine and surfactant protein-B were isolated from serial tracheal aspirates, and their fractional synthetic rate was derived from the 2H and 13C enrichment curves, obtained by gas chromatography mass spectrometry. Disaturated-phosphatidylcholine, surfactant protein-B, and protein concentrations in tracheal aspirates were also measured. Results: 1) Surfactant protein-B turned over at faster rate than disaturated-phosphatidylcholine both in ARDS/ALI patients and in controls. 2) In patients with ARDS/ALI the fractional synthesis rate of disaturated-phosphatidylcholine was 3.1 times higher than in controls (p

Original languageEnglish
Pages (from-to)36
Number of pages1
JournalRespiratory Research
DOIs
Publication statusAccepted/In press - Mar 24 2011

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ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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Simonato, M., Baritussio, A., Ori, C., Vedovelli, L., Rossi, S., Dalla Massara, L., Rizzi, S., Carnielli, V. P., & Cogo, P. E. (Accepted/In press). Disaturated-phosphatidylcholine and Surfactant protein-B turnover in human acute lung injury and in control patients. Respiratory Research, 36. https://doi.org/10.1186/1465-9921-12-36