Discontinuos distribution of IgG oligoclonal bands in cerebrospinal fluid from multiple sclerosis patients

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Abstract

To test the effect of sampling on the detection of immunoglobulin (Ig) cerebrospinal fluid (CSF) abnormalities, we analyzed the first and last 1 ml fraction of 10 ml obtained during a single CSF removal from 27 multiple sclerosis (MS) patients and six patients with other neurological diseases. IgG index, hyperbolic function, and IgG synthesis rate decreased between the first and the last CSF aliquot. Discordant results were found in 4 27 (15%) MS patients. In 2 27 (7.5%) clinically definite MS patients, the number of CSF oligoclonal bands (OCB) decreased between the first and the last fraction. In one of the two patients, the three OCB visualized in the first fraction were not found in the last. We conclude that fractionated sampling may partially account for the absence of OCB in the CSF of some definite MS patients.

Original languageEnglish
Pages (from-to)129-134
Number of pages6
JournalJournal of Neuroimmunology
Volume30
Issue number2-3
DOIs
Publication statusPublished - 1990

Fingerprint

Oligoclonal Bands
Multiple Sclerosis
Cerebrospinal Fluid
Immunoglobulin G
Immunoglobulins

Keywords

  • Cerebrospinal fluid
  • Isoelectric focusing
  • Multiple sclerosis
  • Oligoclonal band

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Neurology
  • Neurology

Cite this

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title = "Discontinuos distribution of IgG oligoclonal bands in cerebrospinal fluid from multiple sclerosis patients",
abstract = "To test the effect of sampling on the detection of immunoglobulin (Ig) cerebrospinal fluid (CSF) abnormalities, we analyzed the first and last 1 ml fraction of 10 ml obtained during a single CSF removal from 27 multiple sclerosis (MS) patients and six patients with other neurological diseases. IgG index, hyperbolic function, and IgG synthesis rate decreased between the first and the last CSF aliquot. Discordant results were found in 4 27 (15{\%}) MS patients. In 2 27 (7.5{\%}) clinically definite MS patients, the number of CSF oligoclonal bands (OCB) decreased between the first and the last fraction. In one of the two patients, the three OCB visualized in the first fraction were not found in the last. We conclude that fractionated sampling may partially account for the absence of OCB in the CSF of some definite MS patients.",
keywords = "Cerebrospinal fluid, Isoelectric focusing, Multiple sclerosis, Oligoclonal band",
author = "Gianvito Martino and Grimaldi, {Luigi M E} and Lucia Moiola and Massimo Filippi and Vittorio Martinelli and Giancarlo Comi and Nicola Canal",
year = "1990",
doi = "10.1016/0165-5728(90)90096-6",
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pages = "129--134",
journal = "Journal of Neuroimmunology",
issn = "0165-5728",
publisher = "Elsevier Science B.V.",
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TY - JOUR

T1 - Discontinuos distribution of IgG oligoclonal bands in cerebrospinal fluid from multiple sclerosis patients

AU - Martino, Gianvito

AU - Grimaldi, Luigi M E

AU - Moiola, Lucia

AU - Filippi, Massimo

AU - Martinelli, Vittorio

AU - Comi, Giancarlo

AU - Canal, Nicola

PY - 1990

Y1 - 1990

N2 - To test the effect of sampling on the detection of immunoglobulin (Ig) cerebrospinal fluid (CSF) abnormalities, we analyzed the first and last 1 ml fraction of 10 ml obtained during a single CSF removal from 27 multiple sclerosis (MS) patients and six patients with other neurological diseases. IgG index, hyperbolic function, and IgG synthesis rate decreased between the first and the last CSF aliquot. Discordant results were found in 4 27 (15%) MS patients. In 2 27 (7.5%) clinically definite MS patients, the number of CSF oligoclonal bands (OCB) decreased between the first and the last fraction. In one of the two patients, the three OCB visualized in the first fraction were not found in the last. We conclude that fractionated sampling may partially account for the absence of OCB in the CSF of some definite MS patients.

AB - To test the effect of sampling on the detection of immunoglobulin (Ig) cerebrospinal fluid (CSF) abnormalities, we analyzed the first and last 1 ml fraction of 10 ml obtained during a single CSF removal from 27 multiple sclerosis (MS) patients and six patients with other neurological diseases. IgG index, hyperbolic function, and IgG synthesis rate decreased between the first and the last CSF aliquot. Discordant results were found in 4 27 (15%) MS patients. In 2 27 (7.5%) clinically definite MS patients, the number of CSF oligoclonal bands (OCB) decreased between the first and the last fraction. In one of the two patients, the three OCB visualized in the first fraction were not found in the last. We conclude that fractionated sampling may partially account for the absence of OCB in the CSF of some definite MS patients.

KW - Cerebrospinal fluid

KW - Isoelectric focusing

KW - Multiple sclerosis

KW - Oligoclonal band

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