Discordance between genotypic and phenotypic drug resistance profiles in human immunodeficiency virus type 1 strains isolated from peripheral blood mononuclear cells

Loredana Sarmati, Emanuele Nicastri, Saverio G. Parisi, Gabriella D'Ettorre, Giorgio Mancino, Pasquale Narciso, Vincenzo Vullo, Massimo Andreoni

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The aim of the study was to analyze the relationship between genotypic and phenotypic drug resistance profiles of human immunodeficiency virus type 1 (HIV-1) strains isolated from patients during double-analogue nucleoside therapy. A drug-resistant HIV strain was isolated from 20 out of 25 patients, with 16 (64%) subjects carrying a virus with multiple drug resistance mutations. The most frequent resistance mutations were M184V (18 isolates) and M41L (7 isolates). Discordance between the genotypic and phenotypic profile for at least one drug was detected in 16 out of 25 strains. Particularly, eight isolates had a discordant genotypic-phenotypic resistance pattern for two drugs and one isolate had such a pattern for three drugs. A genotypic resistance pattern with a phenotypic sensitivity profile was detected in six isolates (four resistant to zidovudine and two resistant to lamivudine). On the other hand for several strains a genotypic pattern of sensitivity pattern to abacavir (10 strains), didanosine (7 strains), stavudine (3 strains), zidovudine (2 strains), and lamivudine (1 strain) with a phenotypic resistance profile was detected. After a follow-up period of 8 months, an impairment of virological and immunological parameters was detected only in subjects with an HIV-1 isolate with a phenotypic resistance profile in despite of the genotypic results. Predicting resistance phenotype from genotypic data has important limitations. Despite the low number of patients and the short follow-up period, this study suggests that during failing therapy with analogue nucleosides, a phenotypic analysis could be performed in spite of an HIV genotypic sensitivity pattern.

Original languageEnglish
Pages (from-to)335-340
Number of pages6
JournalJournal of Clinical Microbiology
Volume40
Issue number2
DOIs
Publication statusPublished - 2002

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Drug Resistance
HIV-1
Blood Cells
Lamivudine
Zidovudine
Nucleosides
Pharmaceutical Preparations
HIV
Stavudine
Didanosine
Mutation
Multiple Drug Resistance
Viruses
Phenotype
Therapeutics

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

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Discordance between genotypic and phenotypic drug resistance profiles in human immunodeficiency virus type 1 strains isolated from peripheral blood mononuclear cells. / Sarmati, Loredana; Nicastri, Emanuele; Parisi, Saverio G.; D'Ettorre, Gabriella; Mancino, Giorgio; Narciso, Pasquale; Vullo, Vincenzo; Andreoni, Massimo.

In: Journal of Clinical Microbiology, Vol. 40, No. 2, 2002, p. 335-340.

Research output: Contribution to journalArticle

Sarmati, Loredana ; Nicastri, Emanuele ; Parisi, Saverio G. ; D'Ettorre, Gabriella ; Mancino, Giorgio ; Narciso, Pasquale ; Vullo, Vincenzo ; Andreoni, Massimo. / Discordance between genotypic and phenotypic drug resistance profiles in human immunodeficiency virus type 1 strains isolated from peripheral blood mononuclear cells. In: Journal of Clinical Microbiology. 2002 ; Vol. 40, No. 2. pp. 335-340.
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