Discovery and initial verification of differentially abundant proteins between multiple sclerosis patients and controls using iTRAQ and SID-SRM

Ann C. Kroksveen, Elise Aasebø, Heidrun Vethe, Vincent Van Pesch, Diego Franciotta, Charlotte E. Teunissen, Rune J. Ulvik, Christian Vedeler, Kjell Morten Myhr, Harald Barsnes, Frode S. Berven

Research output: Contribution to journalArticle

Abstract

In the present study, we aimed to discover cerebrospinal fluid (CSF) proteins with significant abundance difference between early multiple sclerosis patients and controls, and do an initial verification of these proteins using selected reaction monitoring (SRM). iTRAQ and Orbitrap MS were used to compare the CSF proteome of patients with clinically isolated syndrome (CIS) (n = 5), patients with relapsing-remitting multiple sclerosis that had CIS at the time of lumbar puncture (n = 5), and controls with other inflammatory neurological disease (n = 5). Of more than 1200 identified proteins, five proteins were identified with significant abundance difference between the patients and controls. In the initial verification using SRM we analyzed a larger patient and control cohort (n = 132) and also included proteins reported as differentially abundant in multiple sclerosis in the literature. We found significant abundance difference for 11 proteins after verification, of which the five proteins alpha-1-antichymotrypsin, contactin-1, apolipoprotein D, clusterin, and kallikrein-6 were significantly differentially abundant in several of the group comparisons. This initial study form the basis for further biomarker verification studies in even larger sample cohorts, to determine if these proteins have relevance as diagnostic or prognostic biomarkers for multiple sclerosis.

Original languageEnglish
Pages (from-to)312-325
Number of pages14
JournalJournal of Proteomics
Volume78
DOIs
Publication statusPublished - Jan 14 2013

Keywords

  • Cerebrospinal fluid
  • Multiple sclerosis
  • Quantitative mass spectrometry
  • Selected reaction monitoring

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

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    Kroksveen, A. C., Aasebø, E., Vethe, H., Van Pesch, V., Franciotta, D., Teunissen, C. E., Ulvik, R. J., Vedeler, C., Myhr, K. M., Barsnes, H., & Berven, F. S. (2013). Discovery and initial verification of differentially abundant proteins between multiple sclerosis patients and controls using iTRAQ and SID-SRM. Journal of Proteomics, 78, 312-325. https://doi.org/10.1016/j.jprot.2012.09.037