Discrimination and classification of acute lymphoblastic leukemia cells by Raman spectroscopy

Stefano Managò, Carmen Valente, Peppino Mirabelli, Anna Chiara De Luca

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Currently, a combination of technologies is typically required to identify and classify leukemia cells. These methods often lack the specificity and sensitivity necessary for early and accurate diagnosis. Here, we demonstrate the use of Raman spectroscopy to identify normal B cells, collected from healthy patients, and three ALL cell lines (RS4;11, REH and MN60 at different differentiation level, respectively). Raman markers associated with DNA and protein vibrational modes have been identified that exhibit excellent discriminating power for leukemia cell identification. Principal Component Analysis was finally used to confirm the significance of these markers for identify leukemia cells and classifying the data. The obtained results indicate a sorting accuracy of 96% between the three leukemia cell lines.

Original languageEnglish
Title of host publicationOptical Sensors 2015
PublisherSPIE
Volume9506
ISBN (Print)9781628416275
DOIs
Publication statusPublished - 2015
EventOptical Sensors 2015 - Prague, Czech Republic
Duration: Apr 13 2015Apr 16 2015

Other

OtherOptical Sensors 2015
CountryCzech Republic
CityPrague
Period4/13/154/16/15

Fingerprint

leukemias
Raman Spectroscopy
Leukemia
Acute
Discrimination
discrimination
Raman spectroscopy
Cells
Cell
classifying
cells
cultured cells
markers
Sorting
Principal component analysis
DNA
principal components analysis
B Cells
Line
Proteins

Keywords

  • diagnosis
  • leukemia
  • Raman spectroscopy

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Managò, S., Valente, C., Mirabelli, P., & De Luca, A. C. (2015). Discrimination and classification of acute lymphoblastic leukemia cells by Raman spectroscopy. In Optical Sensors 2015 (Vol. 9506). [95060Z] SPIE. https://doi.org/10.1117/12.2179486

Discrimination and classification of acute lymphoblastic leukemia cells by Raman spectroscopy. / Managò, Stefano; Valente, Carmen; Mirabelli, Peppino; De Luca, Anna Chiara.

Optical Sensors 2015. Vol. 9506 SPIE, 2015. 95060Z.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Managò, S, Valente, C, Mirabelli, P & De Luca, AC 2015, Discrimination and classification of acute lymphoblastic leukemia cells by Raman spectroscopy. in Optical Sensors 2015. vol. 9506, 95060Z, SPIE, Optical Sensors 2015, Prague, Czech Republic, 4/13/15. https://doi.org/10.1117/12.2179486
Managò, Stefano ; Valente, Carmen ; Mirabelli, Peppino ; De Luca, Anna Chiara. / Discrimination and classification of acute lymphoblastic leukemia cells by Raman spectroscopy. Optical Sensors 2015. Vol. 9506 SPIE, 2015.
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