TY - JOUR
T1 - Discrimination of glioma patient-derived cells from healthy astrocytes by exploiting Raman spectroscopy
AU - Iturrioz-Rodríguez, Nerea
AU - De Pasquale, Daniele
AU - Fiaschi, Pietro
AU - Ciofani, Gianni
N1 - Funding Information:
The research leading to these results has received funding from AIRC under IG 2020 – ID 24454 – P.I. Gianni Ciofani.
Publisher Copyright:
© 2021 The Authors
PY - 2022/3/15
Y1 - 2022/3/15
N2 - Glioblastoma multiforme (GBM) is one of the most common and aggressive brain tumors. It presents a very bad prognosis with a patients’ overall survival of 12–15 months; treatment failure is mainly ascribable to tumor recurrence. The development of new tools, that could help the precise detection of the tumor border, is thus an urgent need. During the last decades, different vibrational spectroscopy techniques have been developed to distinguish cancer tissue from heathy tissue; in the present work, we compared GBM cells deriving from four patients with healthy human astrocytes using Raman spectroscopy. We have shown that the region between 1000 and 1300 cm−1 is enough informative for this discrimination, indeed highlighting that peaks related to DNA/RNA and cytochrome c are increased in cancer cells. Finally, our model has been able to discriminate cancer cells from healthy cells with an average accuracy of 92.5%. We believe that this study might help to further understand which are the essential Raman peaks exploitable in the detection of cancer cells, with important perspectives under a diagnostic point of view.
AB - Glioblastoma multiforme (GBM) is one of the most common and aggressive brain tumors. It presents a very bad prognosis with a patients’ overall survival of 12–15 months; treatment failure is mainly ascribable to tumor recurrence. The development of new tools, that could help the precise detection of the tumor border, is thus an urgent need. During the last decades, different vibrational spectroscopy techniques have been developed to distinguish cancer tissue from heathy tissue; in the present work, we compared GBM cells deriving from four patients with healthy human astrocytes using Raman spectroscopy. We have shown that the region between 1000 and 1300 cm−1 is enough informative for this discrimination, indeed highlighting that peaks related to DNA/RNA and cytochrome c are increased in cancer cells. Finally, our model has been able to discriminate cancer cells from healthy cells with an average accuracy of 92.5%. We believe that this study might help to further understand which are the essential Raman peaks exploitable in the detection of cancer cells, with important perspectives under a diagnostic point of view.
KW - Diagnosis
KW - Glioblastoma multiforme
KW - PCA-LDA
KW - Raman spectroscopy
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U2 - 10.1016/j.saa.2021.120773
DO - 10.1016/j.saa.2021.120773
M3 - Article
AN - SCOPUS:85121594333
VL - 269
JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
SN - 1386-1425
M1 - 120773
ER -