Disposition of d-fenfluramine in lean and obese rats

Claudia Fracasso, Giovanna Guiso, Silvio Garattini, Silvio Caccia

Research output: Contribution to journalArticle

Abstract

The anorectic drug d-fenfluramine (d-F) was administered as single i.v. doses of 1·25 and 6·25 mg/kg to lean female Sprague-Dawley and lean and obese female Zucker rats. Blood samples were collected serially and analysed by electron capture gasliquid chromatography for d-F and its main metabolite, d-norfenfluramine (d-NF). At the lowest dose the disappearance of d-F followed an apparent first-order process with mean elimination half-life (T1/2) of approximately 2 h in female Sprague-Dawley and 4 h in lean and obese Zucker rats. Mean absolute steady-state volume of distribution (Vss) was the same in the lean female of both strains but total clearance (Cl) was significantly lower in the Zucker rats. Therefore elimination T1/2 of d-F was longer in female Zucker than Sprague-Dawley animals. Obese rats presented lower relative Cl and Vss but no change in absolute Cl and Vss and elimination T1/2 of the drug. Intra- and inter-strain differences were observed in hepatic microsomal protein and P-450 content. As in the case of d-F the elimination T1/2 of d-NF was also longer in Zucker than Sprague-Dawley rats. No differences were observed between lean and obese rats but in all cases the elimination T1/2 of the metabolite was much longer than that of its parent drug. After larger doses (6·25 mg/kg) the kinetics of the drug were not linear. The apparent Cl declined changing the metabolite-to-parent drug ratios in all types of rats, but more evidently in Zucker than Sprague-Dawley rats and in obese than lean animals. Inter- and intra-strain differences in d-F and d-NF kinetics should be considered in neurochemical studies of the drug and extrapolation of data across animal species requires consideration of dose dependence in the rat.

Original languageEnglish
Pages (from-to)45-55
Number of pages11
JournalAppetite
Volume10
Issue number1
DOIs
Publication statusPublished - 1988

ASJC Scopus subject areas

  • Nutrition and Dietetics
  • Psychology(all)
  • Behavioral Neuroscience

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