Disruption of cortical development as a consequence of repetitive pilocarpine-induced status epilepticus in rats

Alexandre Valotta Da Silva, Maria Cristina Regondi, E. A. Cavalheiro, Roberto Spreafico

Research output: Contribution to journalArticlepeer-review


Purpose: The aim of the present study was to observe possible cortical abnormalities after repetitive pilocarpine-induced status epilepticus (SE) in rats during development. Methods: Wistar rats received intraperitoneal injection of pilocarpine hydrochloride 2% (380 mg/kg) at P7, P8, and P9. All experimental rats displayed SE after pilocarpine injections. Rats were killed at P10 and P35, and immunocytochemistry procedures were performed on 50-μm vibratome sections, by using antibodies against nonphosphorylated neurofilament (SMI-311), parvalbumin (PV), calbindin (CB), calretinin (CR), and glutamate decarboxylase (GAD-65). Selected sections were used for the TUNEL method and double-labeling experiments, with different mixtures of the same markers. Results: The major findings of the present work were (a) altered intracortical circuitry development; (b) anticipation of PV immunoreactivity in neocortical interneurons; (c) increased GAD-65 immunoreactivity; and (d) reduced neocortical apoptotic process. Conclusions: From these results, we suggest that previously healthy brain, without genetic abnormalities, might develop an "acquired" disruption of cortical development whose evolution reproduces some characteristics of the childhood epilepsies associated with cognitive impairment.

Original languageEnglish
Pages (from-to)22-30
Number of pages9
Issue numberSUPPL. 5
Publication statusPublished - Jul 2005


  • Apoptosis
  • Early insult
  • Immature brain
  • Interneurons
  • Neocortex

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)


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