Dissecting FMR1, the protein responsible for fragile X syndrome, in its structural and functional domains

Salvatore Adinolfi, Claudia Bagni, Giovanna Musco, Toby Gibson, Lelio Mazzarella, Annalisa Pastore

Research output: Contribution to journalArticle

Abstract

FMR1 is an RNA-binding protein that is either absent or mutated in patients affected by the fragile x syndrome, the most common inherited cause of mental retardation in humans. Sequence analysis of the FMR1 protein has suggested that RNA binding is related to the presence of two K-homologous (KH) modules and an RGG box. However, no attempt has been so far made to map the RNA-binding sites along the protein sequence and to identify possible differential RNA-sequence specificity. In the present article, we describe work done to dissect FMR1 into regions with structurally and functionally distinct properties. A semirational approach was followed to identify four regions: an N-terminal stretch of 200 amino acids, the two KH regions, and a C-terminal stretch. Each region was produced as a recombinant protein, purified, and probed for its state of folding by spectroscopical techniques. Circular dichroism and NMR spectra of the N-terminus show formation of secondary structure with a strong tendency to aggregate. Of the two homologous KH motifs, only the first one is folded whereas the second remains unfolded even when it is extended both N- and C-terminally. The C-terminus is, as expected from its amino acid composition, nonglobular. Binding assays were then performed using the 4-nt homopolymers. Our results show that only the first KH domain but not the second binds to RNA, and provide the first direct evidence for RNA binding of both the N-terminal and the C-terminal regions. RNA binding for the N-terminus could not be predicted from sequence analysis because no known RNA-binding motif is identifiable in this region. Different sequence specificity was observed for the fragments: both the N- terminus of the protein and KH1 bind preferentially to poly-(rG). The C- terminal region, which contains the RGG box, is nonspecific, as it recognizes the bases with comparable affinity. We therefore conclude that FMR1 is a protein with multiple sites of interaction with RNA: sequence specificity is most likely achieved by the whole block that comprises the first ≃400 residues, whereas the C-terminus provides a nonspecific binding surface.

Original languageEnglish
Pages (from-to)1248-1258
Number of pages11
JournalRNA
Volume5
Issue number9
DOIs
Publication statusPublished - Sep 1999

Keywords

  • FMR1
  • Fragile sites
  • Fragile X
  • Modular proteins
  • RNA-binding proteins

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

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