Dissection of human humoral immune response against hepatitis C virus E2 glycoprotein by repertoire cloning and generation of recombinant Fab fragments

Roberto Burioni, Paola Plaisant, Aldo Manzin, Domenico Rosa, Valeria Delli Carri, Francesca Bugli, Laura Solforosi, Sergio Abrignani, Pietro E. Varaldo, Giovanni Fadda, Massimo Clementi

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Demonstration of antibodies inhibiting key viral functions is the basis for the design of an effective vaccine. Dissection of the human antibody response by repertoire cloning may be a powerful means to address this issue. In this study, a panel of human monoclonal recombinant Fab fragments specific for hepatitis C virus (HCV) E2 envelope protein was generated. The selection procedure was designed to select for cross-genotype reactive antibodies. Sequences coding five different human recombinant Fabs specific for the HCV/E2 protein were cloned and characterized. The ability of the cloned antibody fragments to inhibit adhesion of recombinant envelope E2 protein to target cells was assayed. While affinity of the different antibody fragments appeared similar, activity in inhibiting E2 binding to target cells varied considerably from one Fab fragment to another. Two Fabs were not able to inhibit E2 binding at high concentration (40 μg/mL), while three other Fab clones were active in neutralizing 50% of the E2 binding at concentrations ranging from 3 to 0.35 μg/mL.

Original languageEnglish
Pages (from-to)810-814
Number of pages5
JournalHepatology
Volume28
Issue number3
Publication statusPublished - 1998

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Immunoglobulin Fab Fragments
Humoral Immunity
Dissection
Organism Cloning
Immunoglobulin Fragments
Hepacivirus
Viral Envelope Proteins
Antibodies
Antibody Formation
Proteins
Vaccines
Clone Cells
Genotype
Hepatitis C virus glycoprotein E2

ASJC Scopus subject areas

  • Hepatology

Cite this

Dissection of human humoral immune response against hepatitis C virus E2 glycoprotein by repertoire cloning and generation of recombinant Fab fragments. / Burioni, Roberto; Plaisant, Paola; Manzin, Aldo; Rosa, Domenico; Carri, Valeria Delli; Bugli, Francesca; Solforosi, Laura; Abrignani, Sergio; Varaldo, Pietro E.; Fadda, Giovanni; Clementi, Massimo.

In: Hepatology, Vol. 28, No. 3, 1998, p. 810-814.

Research output: Contribution to journalArticle

Burioni, R, Plaisant, P, Manzin, A, Rosa, D, Carri, VD, Bugli, F, Solforosi, L, Abrignani, S, Varaldo, PE, Fadda, G & Clementi, M 1998, 'Dissection of human humoral immune response against hepatitis C virus E2 glycoprotein by repertoire cloning and generation of recombinant Fab fragments', Hepatology, vol. 28, no. 3, pp. 810-814.
Burioni, Roberto ; Plaisant, Paola ; Manzin, Aldo ; Rosa, Domenico ; Carri, Valeria Delli ; Bugli, Francesca ; Solforosi, Laura ; Abrignani, Sergio ; Varaldo, Pietro E. ; Fadda, Giovanni ; Clementi, Massimo. / Dissection of human humoral immune response against hepatitis C virus E2 glycoprotein by repertoire cloning and generation of recombinant Fab fragments. In: Hepatology. 1998 ; Vol. 28, No. 3. pp. 810-814.
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AU - Carri, Valeria Delli

AU - Bugli, Francesca

AU - Solforosi, Laura

AU - Abrignani, Sergio

AU - Varaldo, Pietro E.

AU - Fadda, Giovanni

AU - Clementi, Massimo

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