Distamycin inhibits the binding of a nuclear factor to the -278/-256 upstream sequence of the human HLA-DRα gene

Roberto Gambari, Rafaella Barbieri, Claudio Nastruzzi, Valeria Chiorboli, Giordana Feriotto, Pier Giorgio Natali, Patrizio Giacomini, Federico Arcamone

Research output: Contribution to journalArticle

Abstract

In this study we analyse the effects of the anti-tumor compound distamycin on the binding of nuclear factor(s) to a synthetic oligonucleotide (GTATA/IFN-γ) mimicking a putative regulatory region of the human HLA-DRα gene. This region contains the sequence (GTATA), that is required for nuclear protein binding and is likely to interact with distamycin. The present results, by showing that distamycin inhibits the interaction between nuclear factors and the GTATA/IFN-γ oligonucleotide, suggest that distamycin might alter the binding of transacting factors to cis-elements containing AT/TA sequences. Alterations of nuclear protein binding to specific target sequences could be one of the molecular mechanism(s) by which distamycin exerts its antiproliferative activity on living cells.

Original languageEnglish
Pages (from-to)497-502
Number of pages6
JournalBiochemical Pharmacology
Volume41
Issue number4
DOIs
Publication statusPublished - Feb 15 1991

ASJC Scopus subject areas

  • Pharmacology

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