Distinct morphophenotypic features of chronic B-cell leukaemias identified with CD1c and CD23 antibodies

A. Orazi, G. Cattoretti, N. Polli, D. Delia, F. Rilke

Research output: Contribution to journalArticlepeer-review

Abstract

Morphological criteria usually applied to diagnose various subtypes of B-cell chronic lymphoid leukaemia are largely subjective. Immunophenotyping of 61 relevant cases using a selected panel of monoclonal antibodies (mAb), showed that CD1c and CD23 mAb were able to separate B-cell chronic lymphocytic leukaemia (B-CLL) from other chronic B-cell lymphoproliferative diseases. Lymphocytes of B-CLL were CD1c-, CD23+, whereas those of other types of chronic B-cell leukaemia were CD1c(+/-), CD23-, and CD38(+/-). Non-B-CLL cases had a significantly higher amount of large peroxidase-negative (unstained) cells analyzed with an automated blood cell counter (Technicon H6000). This type of volumetric assessment allowed a separation between typical and 'atypical' B-CLL, which otherwise were both CD1c-, and CD23+. These combinations of phenotypic markers corresponded to well-defined haematopathological entities, conventionally diagnosed on peripheral blood (PB) and bone marrow smears, and on histologic sections of lymph nodes and spleen.

Original languageEnglish
Pages (from-to)28-35
Number of pages8
JournalEuropean Journal of Haematology
Volume47
Issue number1
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Hematology

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