Distinct pathogenic effects of group B coxsackieviruses on human glomerular and tubular kidney cells

Pier Giulio Conaldi, Luigi Biancone, Antonella Bottelli, Antonella De Martino, Giovanni Camussi, Antonio Toniolo

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The six group B coxsackieviruses (CVBs) are highly prevalent human pathogens that cause viremia followed by involvement of different organs. Clinical and experimental evidence suggests that CVBs can induce kidney injury, but the susceptibility of human renal cells to these viruses is unknown. By using pure cultures of human glomerular and tubular cells, we demonstrated that all CVBs are capable of productively infecting renal cells of three different histotypes. Distinct pathogenic effects were observed. Proximal tubular epithelial cells and, to a lesser extent, glomerular podocytes were highly susceptible to CVBs; in both cases, infection led to cytolysis. In contrast, glomerular mesangial cells supported the replication of the six CVBs but failed to develop overt cytopathologic changes. Mesangial cells continued to produce infectious progeny for numerous serial subcultures (i.e., more than 50 days), especially with type 1, 3, 4, and 5 viruses. In the above cells, persistent infection induced the de novo synthesis of platelet-derived growth factor A/B and enhanced the release of transforming growth factor β1/2. These two factors are important mediators of progression from glomerular inflammation to glomerulosclerosis. CVB replication appeared also to impair the phagocytic and contractile activity of mesangial cells. Loss of these properties-which are important in glomerular physiopathology- may contribute to the development of progressive nephropathy. The results show that CVBs induce distinct effects in different types of cultured renal cells and suggest that CVB infections may be associated with both acute and progressive renal injury.

Original languageEnglish
Pages (from-to)9180-9187
Number of pages8
JournalJournal of Virology
Volume71
Issue number12
Publication statusPublished - Dec 1997

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Human Enterovirus B
Enterovirus
group effect
kidney cells
Mesangial Cells
Kidney
Infection
cells
Proto-Oncogene Proteins c-sis
Viruses
Podocytes
kidneys
infection
Viremia
cytolysis
Transforming Growth Factors
platelet-derived growth factor
transforming growth factors
viruses
Acute Kidney Injury

ASJC Scopus subject areas

  • Immunology

Cite this

Conaldi, P. G., Biancone, L., Bottelli, A., De Martino, A., Camussi, G., & Toniolo, A. (1997). Distinct pathogenic effects of group B coxsackieviruses on human glomerular and tubular kidney cells. Journal of Virology, 71(12), 9180-9187.

Distinct pathogenic effects of group B coxsackieviruses on human glomerular and tubular kidney cells. / Conaldi, Pier Giulio; Biancone, Luigi; Bottelli, Antonella; De Martino, Antonella; Camussi, Giovanni; Toniolo, Antonio.

In: Journal of Virology, Vol. 71, No. 12, 12.1997, p. 9180-9187.

Research output: Contribution to journalArticle

Conaldi, PG, Biancone, L, Bottelli, A, De Martino, A, Camussi, G & Toniolo, A 1997, 'Distinct pathogenic effects of group B coxsackieviruses on human glomerular and tubular kidney cells', Journal of Virology, vol. 71, no. 12, pp. 9180-9187.
Conaldi PG, Biancone L, Bottelli A, De Martino A, Camussi G, Toniolo A. Distinct pathogenic effects of group B coxsackieviruses on human glomerular and tubular kidney cells. Journal of Virology. 1997 Dec;71(12):9180-9187.
Conaldi, Pier Giulio ; Biancone, Luigi ; Bottelli, Antonella ; De Martino, Antonella ; Camussi, Giovanni ; Toniolo, Antonio. / Distinct pathogenic effects of group B coxsackieviruses on human glomerular and tubular kidney cells. In: Journal of Virology. 1997 ; Vol. 71, No. 12. pp. 9180-9187.
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