Distinct proteomic profiles characterise non-erosive from erosive reflux disease

C. Calabrese, V. Marzano, A. Urbani, G. Lazzarini, M. C. Valerii, G. Liguori, S. Di Molfetta, F. Rizzello, P. Gionchetti, M. Campieri, E. Spisni

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background Erosive reflux disease (ERD) and non-erosive reflux disease (NERD) are often regarded as part of the spectrum of the same disease. Aim To elucidate molecular features that characterise NERD and ERD at the protein level. Methods A total of 56 consecutive subjects were enrolled: 10 healthy subjects, 24 with NERD and 22 with ERD. Eight specimens were taken from macroscopically normal mucosa at 5 cm of gastro-oesophageal junction. Four were processed for the proteins extraction and four for evaluation using haematoxylin-eosin and immunohistochemistry. We used shotgun proteomics to identify tentative disease molecular features for ERD or NERD. Candidate distinctive proteins were verified using immunohistochemistry. Results Shotgun proteomics analysis revealed 33 differentially expressed proteins in NERD vs. ERD samples, involved in cellular proliferation, keratinisation, stress responses and sugar metabolism. Based on a gene ontology meta-analysis, seven of them were further analysed using Western blotting (WB) and four also using immunohistochemistry. We identified novel candidate disease molecular features for GERD and few distinctive proteins to discriminate NERD and ERD. In particular, Transitional Endoplasmic Reticulum ATPase (TER ATPase), GAPDH, Alpha 1 Acid Glycoprotein 1, Annexin A1, Calmodulin and 14-3-3 proteins were confirmed at WB analysis. Conclusions Non-erosive reflux disease and ERD are distinct disease entities at the protein level. This study proposes an array of candidate biomarkers possibly useful to discriminate between NERD and ERD.

Original languageEnglish
Pages (from-to)982-993
Number of pages12
JournalAlimentary Pharmacology and Therapeutics
Volume34
Issue number8
DOIs
Publication statusPublished - Oct 2011

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Proteomics
Proteins
Immunohistochemistry
Firearms
Western Blotting
Annexin A1
14-3-3 Proteins
Orosomucoid
Gene Ontology
Calmodulin
Hematoxylin
Eosine Yellowish-(YS)
Gastroesophageal Reflux
Endoplasmic Reticulum

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Distinct proteomic profiles characterise non-erosive from erosive reflux disease. / Calabrese, C.; Marzano, V.; Urbani, A.; Lazzarini, G.; Valerii, M. C.; Liguori, G.; Di Molfetta, S.; Rizzello, F.; Gionchetti, P.; Campieri, M.; Spisni, E.

In: Alimentary Pharmacology and Therapeutics, Vol. 34, No. 8, 10.2011, p. 982-993.

Research output: Contribution to journalArticle

Calabrese, C, Marzano, V, Urbani, A, Lazzarini, G, Valerii, MC, Liguori, G, Di Molfetta, S, Rizzello, F, Gionchetti, P, Campieri, M & Spisni, E 2011, 'Distinct proteomic profiles characterise non-erosive from erosive reflux disease', Alimentary Pharmacology and Therapeutics, vol. 34, no. 8, pp. 982-993. https://doi.org/10.1111/j.1365-2036.2011.04801.x
Calabrese, C. ; Marzano, V. ; Urbani, A. ; Lazzarini, G. ; Valerii, M. C. ; Liguori, G. ; Di Molfetta, S. ; Rizzello, F. ; Gionchetti, P. ; Campieri, M. ; Spisni, E. / Distinct proteomic profiles characterise non-erosive from erosive reflux disease. In: Alimentary Pharmacology and Therapeutics. 2011 ; Vol. 34, No. 8. pp. 982-993.
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N2 - Background Erosive reflux disease (ERD) and non-erosive reflux disease (NERD) are often regarded as part of the spectrum of the same disease. Aim To elucidate molecular features that characterise NERD and ERD at the protein level. Methods A total of 56 consecutive subjects were enrolled: 10 healthy subjects, 24 with NERD and 22 with ERD. Eight specimens were taken from macroscopically normal mucosa at 5 cm of gastro-oesophageal junction. Four were processed for the proteins extraction and four for evaluation using haematoxylin-eosin and immunohistochemistry. We used shotgun proteomics to identify tentative disease molecular features for ERD or NERD. Candidate distinctive proteins were verified using immunohistochemistry. Results Shotgun proteomics analysis revealed 33 differentially expressed proteins in NERD vs. ERD samples, involved in cellular proliferation, keratinisation, stress responses and sugar metabolism. Based on a gene ontology meta-analysis, seven of them were further analysed using Western blotting (WB) and four also using immunohistochemistry. We identified novel candidate disease molecular features for GERD and few distinctive proteins to discriminate NERD and ERD. In particular, Transitional Endoplasmic Reticulum ATPase (TER ATPase), GAPDH, Alpha 1 Acid Glycoprotein 1, Annexin A1, Calmodulin and 14-3-3 proteins were confirmed at WB analysis. Conclusions Non-erosive reflux disease and ERD are distinct disease entities at the protein level. This study proposes an array of candidate biomarkers possibly useful to discriminate between NERD and ERD.

AB - Background Erosive reflux disease (ERD) and non-erosive reflux disease (NERD) are often regarded as part of the spectrum of the same disease. Aim To elucidate molecular features that characterise NERD and ERD at the protein level. Methods A total of 56 consecutive subjects were enrolled: 10 healthy subjects, 24 with NERD and 22 with ERD. Eight specimens were taken from macroscopically normal mucosa at 5 cm of gastro-oesophageal junction. Four were processed for the proteins extraction and four for evaluation using haematoxylin-eosin and immunohistochemistry. We used shotgun proteomics to identify tentative disease molecular features for ERD or NERD. Candidate distinctive proteins were verified using immunohistochemistry. Results Shotgun proteomics analysis revealed 33 differentially expressed proteins in NERD vs. ERD samples, involved in cellular proliferation, keratinisation, stress responses and sugar metabolism. Based on a gene ontology meta-analysis, seven of them were further analysed using Western blotting (WB) and four also using immunohistochemistry. We identified novel candidate disease molecular features for GERD and few distinctive proteins to discriminate NERD and ERD. In particular, Transitional Endoplasmic Reticulum ATPase (TER ATPase), GAPDH, Alpha 1 Acid Glycoprotein 1, Annexin A1, Calmodulin and 14-3-3 proteins were confirmed at WB analysis. Conclusions Non-erosive reflux disease and ERD are distinct disease entities at the protein level. This study proposes an array of candidate biomarkers possibly useful to discriminate between NERD and ERD.

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