Distinctin improves the efficacies of glycopeptides and betalactams against staphylococcal biofilm in an experimental model of central venous catheter infection

Andrea Giacometti, Oscar Cirioni, Roberto Ghiselli, Fiorenza Orlando, Carmela Silvestri, Giovanni Renzone, Irma Testa, Federico Mocchegiani, Agnese Della Vittoria, Vittorio Saba, Andrea Scaloni, Giorgio Scalise

Research output: Contribution to journalArticle

Abstract

The ability of microorganisms to adhere to medical implants is a problem of high visibility and has been focused in numerous investigations. To assess the efficacy of distinctin and conventional antibiotics in the treatment of central venous catheter in vitro and in vivo studies were performed. The in vitro susceptibility assay was performed against S. aureus biofilms developed on 96-well polystyrene tissue culture plates. Efficacy studies were performed in a rat model of CVC infection. Twenty-four hours after implantation, the catheters were filled with distinctin. Thirty minutes later, rats were challenged via the CVC with S. aureus. Administration of antibiotics into the CVC at a concentration equal to the MBC for adherent cells, or at 1024 μg/mL began 24 h later. The killing activities of all antibiotics against adherent bacteria were at least four- to eightfold lower than against freely growing cells. When antibiotics were used in distinctin pretreated wells, they showed a significant increase of activity. The in vivo studies showed that when CVCs were pretreated with distinctin biofilm bacterial load was further decreased to 101 CFU/mL and bacteremia was not detected. Distinctin displays potential as an adjunctive agent to antibiotics in the treatment of CVC-related infections.

Original languageEnglish
Pages (from-to)233-239
Number of pages7
JournalJournal of Biomedical Materials Research - Part A
Volume81
Issue number1
DOIs
Publication statusPublished - Apr 2007

Keywords

  • Antibiotic-lock technique
  • Antimicrobial peptides
  • Biofilm
  • CVC
  • Distinctin

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

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    Giacometti, A., Cirioni, O., Ghiselli, R., Orlando, F., Silvestri, C., Renzone, G., Testa, I., Mocchegiani, F., Della Vittoria, A., Saba, V., Scaloni, A., & Scalise, G. (2007). Distinctin improves the efficacies of glycopeptides and betalactams against staphylococcal biofilm in an experimental model of central venous catheter infection. Journal of Biomedical Materials Research - Part A, 81(1), 233-239. https://doi.org/10.1002/jbm.a.30982