Abstract
BACKGROUND: Oral verrucous carcinomas (OVCs) are characterized by better prognosis than oral squamous cell carcinomas (OSCCs). Because chromosomal instability (CIN) in solid tumors is indicative of prognosis, this study investigated whether OVCs and OSCCs were characterized by differences in CIN biomarkers. METHODS: Fresh or frozen multiple tissue samples were submitted to high-resolution DNA flow cytometry (hr DNA-FCM). RESULTS: DNA aneuploid sublines were detected in 6 of 9 OVCs (66.7%) and in 20 of 25 OSCCs (80.0%). Multiple DNA aneuploid sublines were observed, respectively, in 2 of 6 (33.3%) DNA aneuploid OVCs and in 14 of 20 (70%) DNA aneuploid OSCCs (P =.163). OVCs were mainly characterized by DNA Index (DI) values in the near-diploid region (DI≠1 and DI <1.4), whereas aneuploid OSCCs carried most frequently multiple aneuploid sublines with high DI values (DI 1.4). DNA near-diploid and high aneuploid sublines were, respectively, 87.5% and 12.5% for the OVCs versus 30% and 70% for the OSCCs (P =.004). CONCLUSIONS: Present data suggest that OVCs are characterized by a lower degree of CIN and tumor heterogeneity than OSCCs, such that they appear as "frozen" in an early stage of DNA near-diploid aneuploidy, as previously observed for oral preneoplastic lesions. These DI characteristics, which can easily be obtained by hr DNA-FCM, appear to reflect the well-known differences in aggressiveness and prognosis of OVCs and OSCCs. Cancer
| Original language | English |
|---|---|
| Pages (from-to) | 5052-5057 |
| Number of pages | 6 |
| Journal | Cancer |
| Volume | 117 |
| Issue number | 22 |
| DOIs | |
| Publication status | Published - Nov 15 2011 |
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Keywords
- chromosomal instability
- DNA aneuploidy
- flow cytometry
- oral squamous cell carcinoma
- oral verrucous carcinoma
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Distinctive chromosomal instability patterns in oral verrucous and squamous cell carcinomas detected by high-resolution DNA flow cytometry. / Pentenero, Monica; Donadini, Alessandra; Di Nallo, Emanuela; Maffei, Massimo; Marino, Roberto; Familiari, Ubaldo; Broccoletti, Roberto; Castagnola, Patrizio; Gandolfo, Sergio; Giaretti, Walter.
In: Cancer, Vol. 117, No. 22, 15.11.2011, p. 5052-5057.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Distinctive chromosomal instability patterns in oral verrucous and squamous cell carcinomas detected by high-resolution DNA flow cytometry
AU - Pentenero, Monica
AU - Donadini, Alessandra
AU - Di Nallo, Emanuela
AU - Maffei, Massimo
AU - Marino, Roberto
AU - Familiari, Ubaldo
AU - Broccoletti, Roberto
AU - Castagnola, Patrizio
AU - Gandolfo, Sergio
AU - Giaretti, Walter
PY - 2011/11/15
Y1 - 2011/11/15
N2 - BACKGROUND: Oral verrucous carcinomas (OVCs) are characterized by better prognosis than oral squamous cell carcinomas (OSCCs). Because chromosomal instability (CIN) in solid tumors is indicative of prognosis, this study investigated whether OVCs and OSCCs were characterized by differences in CIN biomarkers. METHODS: Fresh or frozen multiple tissue samples were submitted to high-resolution DNA flow cytometry (hr DNA-FCM). RESULTS: DNA aneuploid sublines were detected in 6 of 9 OVCs (66.7%) and in 20 of 25 OSCCs (80.0%). Multiple DNA aneuploid sublines were observed, respectively, in 2 of 6 (33.3%) DNA aneuploid OVCs and in 14 of 20 (70%) DNA aneuploid OSCCs (P =.163). OVCs were mainly characterized by DNA Index (DI) values in the near-diploid region (DI≠1 and DI <1.4), whereas aneuploid OSCCs carried most frequently multiple aneuploid sublines with high DI values (DI 1.4). DNA near-diploid and high aneuploid sublines were, respectively, 87.5% and 12.5% for the OVCs versus 30% and 70% for the OSCCs (P =.004). CONCLUSIONS: Present data suggest that OVCs are characterized by a lower degree of CIN and tumor heterogeneity than OSCCs, such that they appear as "frozen" in an early stage of DNA near-diploid aneuploidy, as previously observed for oral preneoplastic lesions. These DI characteristics, which can easily be obtained by hr DNA-FCM, appear to reflect the well-known differences in aggressiveness and prognosis of OVCs and OSCCs. Cancer
AB - BACKGROUND: Oral verrucous carcinomas (OVCs) are characterized by better prognosis than oral squamous cell carcinomas (OSCCs). Because chromosomal instability (CIN) in solid tumors is indicative of prognosis, this study investigated whether OVCs and OSCCs were characterized by differences in CIN biomarkers. METHODS: Fresh or frozen multiple tissue samples were submitted to high-resolution DNA flow cytometry (hr DNA-FCM). RESULTS: DNA aneuploid sublines were detected in 6 of 9 OVCs (66.7%) and in 20 of 25 OSCCs (80.0%). Multiple DNA aneuploid sublines were observed, respectively, in 2 of 6 (33.3%) DNA aneuploid OVCs and in 14 of 20 (70%) DNA aneuploid OSCCs (P =.163). OVCs were mainly characterized by DNA Index (DI) values in the near-diploid region (DI≠1 and DI <1.4), whereas aneuploid OSCCs carried most frequently multiple aneuploid sublines with high DI values (DI 1.4). DNA near-diploid and high aneuploid sublines were, respectively, 87.5% and 12.5% for the OVCs versus 30% and 70% for the OSCCs (P =.004). CONCLUSIONS: Present data suggest that OVCs are characterized by a lower degree of CIN and tumor heterogeneity than OSCCs, such that they appear as "frozen" in an early stage of DNA near-diploid aneuploidy, as previously observed for oral preneoplastic lesions. These DI characteristics, which can easily be obtained by hr DNA-FCM, appear to reflect the well-known differences in aggressiveness and prognosis of OVCs and OSCCs. Cancer
KW - chromosomal instability
KW - DNA aneuploidy
KW - flow cytometry
KW - oral squamous cell carcinoma
KW - oral verrucous carcinoma
UR - http://www.scopus.com/inward/record.url?scp=80555155575&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80555155575&partnerID=8YFLogxK
U2 - 10.1002/cncr.26126
DO - 10.1002/cncr.26126
M3 - Article
C2 - 21590686
AN - SCOPUS:80555155575
VL - 117
SP - 5052
EP - 5057
JO - Cancer
JF - Cancer
SN - 0008-543X
IS - 22
ER -