Distinctive chromosomal instability patterns in oral verrucous and squamous cell carcinomas detected by high-resolution DNA flow cytometry

Monica Pentenero, Alessandra Donadini, Emanuela Di Nallo, Massimo Maffei, Roberto Marino, Ubaldo Familiari, Roberto Broccoletti, Patrizio Castagnola, Sergio Gandolfo, Walter Giaretti

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Abstract

BACKGROUND: Oral verrucous carcinomas (OVCs) are characterized by better prognosis than oral squamous cell carcinomas (OSCCs). Because chromosomal instability (CIN) in solid tumors is indicative of prognosis, this study investigated whether OVCs and OSCCs were characterized by differences in CIN biomarkers. METHODS: Fresh or frozen multiple tissue samples were submitted to high-resolution DNA flow cytometry (hr DNA-FCM). RESULTS: DNA aneuploid sublines were detected in 6 of 9 OVCs (66.7%) and in 20 of 25 OSCCs (80.0%). Multiple DNA aneuploid sublines were observed, respectively, in 2 of 6 (33.3%) DNA aneuploid OVCs and in 14 of 20 (70%) DNA aneuploid OSCCs (P =.163). OVCs were mainly characterized by DNA Index (DI) values in the near-diploid region (DI≠1 and DI <1.4), whereas aneuploid OSCCs carried most frequently multiple aneuploid sublines with high DI values (DI 1.4). DNA near-diploid and high aneuploid sublines were, respectively, 87.5% and 12.5% for the OVCs versus 30% and 70% for the OSCCs (P =.004). CONCLUSIONS: Present data suggest that OVCs are characterized by a lower degree of CIN and tumor heterogeneity than OSCCs, such that they appear as "frozen" in an early stage of DNA near-diploid aneuploidy, as previously observed for oral preneoplastic lesions. These DI characteristics, which can easily be obtained by hr DNA-FCM, appear to reflect the well-known differences in aggressiveness and prognosis of OVCs and OSCCs. Cancer

Original languageEnglish
Pages (from-to)5052-5057
Number of pages6
JournalCancer
Volume117
Issue number22
DOIs
Publication statusPublished - Nov 15 2011

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Chromosomal Instability
Verrucous Carcinoma
Squamous Cell Carcinoma
Flow Cytometry
Aneuploidy
DNA
Diploidy
Neoplasms

Keywords

  • chromosomal instability
  • DNA aneuploidy
  • flow cytometry
  • oral squamous cell carcinoma
  • oral verrucous carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Pentenero, M., Donadini, A., Di Nallo, E., Maffei, M., Marino, R., Familiari, U., ... Giaretti, W. (2011). Distinctive chromosomal instability patterns in oral verrucous and squamous cell carcinomas detected by high-resolution DNA flow cytometry. Cancer, 117(22), 5052-5057. https://doi.org/10.1002/cncr.26126

Distinctive chromosomal instability patterns in oral verrucous and squamous cell carcinomas detected by high-resolution DNA flow cytometry. / Pentenero, Monica; Donadini, Alessandra; Di Nallo, Emanuela; Maffei, Massimo; Marino, Roberto; Familiari, Ubaldo; Broccoletti, Roberto; Castagnola, Patrizio; Gandolfo, Sergio; Giaretti, Walter.

In: Cancer, Vol. 117, No. 22, 15.11.2011, p. 5052-5057.

Research output: Contribution to journalArticle

Pentenero, M, Donadini, A, Di Nallo, E, Maffei, M, Marino, R, Familiari, U, Broccoletti, R, Castagnola, P, Gandolfo, S & Giaretti, W 2011, 'Distinctive chromosomal instability patterns in oral verrucous and squamous cell carcinomas detected by high-resolution DNA flow cytometry', Cancer, vol. 117, no. 22, pp. 5052-5057. https://doi.org/10.1002/cncr.26126
Pentenero, Monica ; Donadini, Alessandra ; Di Nallo, Emanuela ; Maffei, Massimo ; Marino, Roberto ; Familiari, Ubaldo ; Broccoletti, Roberto ; Castagnola, Patrizio ; Gandolfo, Sergio ; Giaretti, Walter. / Distinctive chromosomal instability patterns in oral verrucous and squamous cell carcinomas detected by high-resolution DNA flow cytometry. In: Cancer. 2011 ; Vol. 117, No. 22. pp. 5052-5057.
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abstract = "BACKGROUND: Oral verrucous carcinomas (OVCs) are characterized by better prognosis than oral squamous cell carcinomas (OSCCs). Because chromosomal instability (CIN) in solid tumors is indicative of prognosis, this study investigated whether OVCs and OSCCs were characterized by differences in CIN biomarkers. METHODS: Fresh or frozen multiple tissue samples were submitted to high-resolution DNA flow cytometry (hr DNA-FCM). RESULTS: DNA aneuploid sublines were detected in 6 of 9 OVCs (66.7{\%}) and in 20 of 25 OSCCs (80.0{\%}). Multiple DNA aneuploid sublines were observed, respectively, in 2 of 6 (33.3{\%}) DNA aneuploid OVCs and in 14 of 20 (70{\%}) DNA aneuploid OSCCs (P =.163). OVCs were mainly characterized by DNA Index (DI) values in the near-diploid region (DI≠1 and DI <1.4), whereas aneuploid OSCCs carried most frequently multiple aneuploid sublines with high DI values (DI 1.4). DNA near-diploid and high aneuploid sublines were, respectively, 87.5{\%} and 12.5{\%} for the OVCs versus 30{\%} and 70{\%} for the OSCCs (P =.004). CONCLUSIONS: Present data suggest that OVCs are characterized by a lower degree of CIN and tumor heterogeneity than OSCCs, such that they appear as {"}frozen{"} in an early stage of DNA near-diploid aneuploidy, as previously observed for oral preneoplastic lesions. These DI characteristics, which can easily be obtained by hr DNA-FCM, appear to reflect the well-known differences in aggressiveness and prognosis of OVCs and OSCCs. Cancer",
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AU - Pentenero, Monica

AU - Donadini, Alessandra

AU - Di Nallo, Emanuela

AU - Maffei, Massimo

AU - Marino, Roberto

AU - Familiari, Ubaldo

AU - Broccoletti, Roberto

AU - Castagnola, Patrizio

AU - Gandolfo, Sergio

AU - Giaretti, Walter

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N2 - BACKGROUND: Oral verrucous carcinomas (OVCs) are characterized by better prognosis than oral squamous cell carcinomas (OSCCs). Because chromosomal instability (CIN) in solid tumors is indicative of prognosis, this study investigated whether OVCs and OSCCs were characterized by differences in CIN biomarkers. METHODS: Fresh or frozen multiple tissue samples were submitted to high-resolution DNA flow cytometry (hr DNA-FCM). RESULTS: DNA aneuploid sublines were detected in 6 of 9 OVCs (66.7%) and in 20 of 25 OSCCs (80.0%). Multiple DNA aneuploid sublines were observed, respectively, in 2 of 6 (33.3%) DNA aneuploid OVCs and in 14 of 20 (70%) DNA aneuploid OSCCs (P =.163). OVCs were mainly characterized by DNA Index (DI) values in the near-diploid region (DI≠1 and DI <1.4), whereas aneuploid OSCCs carried most frequently multiple aneuploid sublines with high DI values (DI 1.4). DNA near-diploid and high aneuploid sublines were, respectively, 87.5% and 12.5% for the OVCs versus 30% and 70% for the OSCCs (P =.004). CONCLUSIONS: Present data suggest that OVCs are characterized by a lower degree of CIN and tumor heterogeneity than OSCCs, such that they appear as "frozen" in an early stage of DNA near-diploid aneuploidy, as previously observed for oral preneoplastic lesions. These DI characteristics, which can easily be obtained by hr DNA-FCM, appear to reflect the well-known differences in aggressiveness and prognosis of OVCs and OSCCs. Cancer

AB - BACKGROUND: Oral verrucous carcinomas (OVCs) are characterized by better prognosis than oral squamous cell carcinomas (OSCCs). Because chromosomal instability (CIN) in solid tumors is indicative of prognosis, this study investigated whether OVCs and OSCCs were characterized by differences in CIN biomarkers. METHODS: Fresh or frozen multiple tissue samples were submitted to high-resolution DNA flow cytometry (hr DNA-FCM). RESULTS: DNA aneuploid sublines were detected in 6 of 9 OVCs (66.7%) and in 20 of 25 OSCCs (80.0%). Multiple DNA aneuploid sublines were observed, respectively, in 2 of 6 (33.3%) DNA aneuploid OVCs and in 14 of 20 (70%) DNA aneuploid OSCCs (P =.163). OVCs were mainly characterized by DNA Index (DI) values in the near-diploid region (DI≠1 and DI <1.4), whereas aneuploid OSCCs carried most frequently multiple aneuploid sublines with high DI values (DI 1.4). DNA near-diploid and high aneuploid sublines were, respectively, 87.5% and 12.5% for the OVCs versus 30% and 70% for the OSCCs (P =.004). CONCLUSIONS: Present data suggest that OVCs are characterized by a lower degree of CIN and tumor heterogeneity than OSCCs, such that they appear as "frozen" in an early stage of DNA near-diploid aneuploidy, as previously observed for oral preneoplastic lesions. These DI characteristics, which can easily be obtained by hr DNA-FCM, appear to reflect the well-known differences in aggressiveness and prognosis of OVCs and OSCCs. Cancer

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KW - oral squamous cell carcinoma

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