Abstract
Purpose: We investigated the distribution of cannabinoid receptor subtypes 1 and 2 in the detrusor of different species and studied the effects of cannabinoid receptor 1 and 2 agonists on bladder function. Materials and Methods: Cannabinoid receptor 1 and 2 expression was studied with Western blot and immunohistochemistry in rat, monkey and human detrusors. Co-staining was done for markers of sensory nerves using calcitonin gene-related peptide (Euro-Diagnostica, Malmö, Sweden) and transient receptor potential vanilloid 1, and for cholinergic nerves using VAChT (Santa Cruz Biotechnology, Santa Cruz, California). Actions of the endogenous cannabinoid receptor-1 and 2 agonist anandamide (Sigma®), and the cannabinoid receptor 1 and 2 agonist CP55,940 (Sigma) on isolated detrusor and during cystometry in conscious rats were recorded. Results: Higher expression of cannabinoid receptor 2 but not cannabinoid receptor 1 was noted in the mucosa than in the detrusor. Compared to the detrusor larger amounts of cannabinoid receptor 2 containing nerves that also expressed transient receptor potential vanilloid 1 or calcitonin gene-related peptide were observed in the suburothelium. Nerve fibers containing cannabinoid receptor 2 and VAChT were located in the detrusor. Neither anandamide nor CP55,940 affected isolated detrusor carbachol (Sigma) contractions. Nerve contractions were enhanced by 10 μM anandamide and decreased by 10 μM CP55,940 (p
| Original language | English |
|---|---|
| Pages (from-to) | 1939-1948 |
| Number of pages | 10 |
| Journal | Journal of Urology |
| Volume | 181 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 2009 |
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Keywords
- anandamide
- bladder
- cannabinoid
- multiple sclerosis
- receptors
- urination
ASJC Scopus subject areas
- Urology
Cite this
Distribution and Function of Cannabinoid Receptors 1 and 2 in the Rat, Monkey and Human Bladder. / Gratzke, Christian; Streng, Tomi; Park, Anthony; Christ, George; Stief, Christian G.; Hedlund, Petter; Andersson, Karl Erik.
In: Journal of Urology, Vol. 181, No. 4, 04.2009, p. 1939-1948.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Distribution and Function of Cannabinoid Receptors 1 and 2 in the Rat, Monkey and Human Bladder
AU - Gratzke, Christian
AU - Streng, Tomi
AU - Park, Anthony
AU - Christ, George
AU - Stief, Christian G.
AU - Hedlund, Petter
AU - Andersson, Karl Erik
PY - 2009/4
Y1 - 2009/4
N2 - Purpose: We investigated the distribution of cannabinoid receptor subtypes 1 and 2 in the detrusor of different species and studied the effects of cannabinoid receptor 1 and 2 agonists on bladder function. Materials and Methods: Cannabinoid receptor 1 and 2 expression was studied with Western blot and immunohistochemistry in rat, monkey and human detrusors. Co-staining was done for markers of sensory nerves using calcitonin gene-related peptide (Euro-Diagnostica, Malmö, Sweden) and transient receptor potential vanilloid 1, and for cholinergic nerves using VAChT (Santa Cruz Biotechnology, Santa Cruz, California). Actions of the endogenous cannabinoid receptor-1 and 2 agonist anandamide (Sigma®), and the cannabinoid receptor 1 and 2 agonist CP55,940 (Sigma) on isolated detrusor and during cystometry in conscious rats were recorded. Results: Higher expression of cannabinoid receptor 2 but not cannabinoid receptor 1 was noted in the mucosa than in the detrusor. Compared to the detrusor larger amounts of cannabinoid receptor 2 containing nerves that also expressed transient receptor potential vanilloid 1 or calcitonin gene-related peptide were observed in the suburothelium. Nerve fibers containing cannabinoid receptor 2 and VAChT were located in the detrusor. Neither anandamide nor CP55,940 affected isolated detrusor carbachol (Sigma) contractions. Nerve contractions were enhanced by 10 μM anandamide and decreased by 10 μM CP55,940 (p
AB - Purpose: We investigated the distribution of cannabinoid receptor subtypes 1 and 2 in the detrusor of different species and studied the effects of cannabinoid receptor 1 and 2 agonists on bladder function. Materials and Methods: Cannabinoid receptor 1 and 2 expression was studied with Western blot and immunohistochemistry in rat, monkey and human detrusors. Co-staining was done for markers of sensory nerves using calcitonin gene-related peptide (Euro-Diagnostica, Malmö, Sweden) and transient receptor potential vanilloid 1, and for cholinergic nerves using VAChT (Santa Cruz Biotechnology, Santa Cruz, California). Actions of the endogenous cannabinoid receptor-1 and 2 agonist anandamide (Sigma®), and the cannabinoid receptor 1 and 2 agonist CP55,940 (Sigma) on isolated detrusor and during cystometry in conscious rats were recorded. Results: Higher expression of cannabinoid receptor 2 but not cannabinoid receptor 1 was noted in the mucosa than in the detrusor. Compared to the detrusor larger amounts of cannabinoid receptor 2 containing nerves that also expressed transient receptor potential vanilloid 1 or calcitonin gene-related peptide were observed in the suburothelium. Nerve fibers containing cannabinoid receptor 2 and VAChT were located in the detrusor. Neither anandamide nor CP55,940 affected isolated detrusor carbachol (Sigma) contractions. Nerve contractions were enhanced by 10 μM anandamide and decreased by 10 μM CP55,940 (p
KW - anandamide
KW - bladder
KW - cannabinoid
KW - multiple sclerosis
KW - receptors
KW - urination
UR - http://www.scopus.com/inward/record.url?scp=62049084948&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=62049084948&partnerID=8YFLogxK
U2 - 10.1016/j.juro.2008.11.079
DO - 10.1016/j.juro.2008.11.079
M3 - Article
VL - 181
SP - 1939
EP - 1948
JO - Journal of Urology
JF - Journal of Urology
SN - 0022-5347
IS - 4
ER -