Distribution and Function of Cannabinoid Receptors 1 and 2 in the Rat, Monkey and Human Bladder

Christian Gratzke, Tomi Streng, Anthony Park, George Christ, Christian G. Stief, Petter Hedlund, Karl Erik Andersson

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Purpose: We investigated the distribution of cannabinoid receptor subtypes 1 and 2 in the detrusor of different species and studied the effects of cannabinoid receptor 1 and 2 agonists on bladder function. Materials and Methods: Cannabinoid receptor 1 and 2 expression was studied with Western blot and immunohistochemistry in rat, monkey and human detrusors. Co-staining was done for markers of sensory nerves using calcitonin gene-related peptide (Euro-Diagnostica, Malmö, Sweden) and transient receptor potential vanilloid 1, and for cholinergic nerves using VAChT (Santa Cruz Biotechnology, Santa Cruz, California). Actions of the endogenous cannabinoid receptor-1 and 2 agonist anandamide (Sigma®), and the cannabinoid receptor 1 and 2 agonist CP55,940 (Sigma) on isolated detrusor and during cystometry in conscious rats were recorded. Results: Higher expression of cannabinoid receptor 2 but not cannabinoid receptor 1 was noted in the mucosa than in the detrusor. Compared to the detrusor larger amounts of cannabinoid receptor 2 containing nerves that also expressed transient receptor potential vanilloid 1 or calcitonin gene-related peptide were observed in the suburothelium. Nerve fibers containing cannabinoid receptor 2 and VAChT were located in the detrusor. Neither anandamide nor CP55,940 affected isolated detrusor carbachol (Sigma) contractions. Nerve contractions were enhanced by 10 μM anandamide and decreased by 10 μM CP55,940 (p

Original languageEnglish
Pages (from-to)1939-1948
Number of pages10
JournalJournal of Urology
Volume181
Issue number4
DOIs
Publication statusPublished - Apr 2009

Fingerprint

Cannabinoid Receptors
Haplorhini
Urinary Bladder
Calcitonin Gene-Related Peptide
Carbachol
Biotechnology
Nerve Fibers
Sweden
Cholinergic Agents
Mucous Membrane
Western Blotting
Immunohistochemistry
Staining and Labeling

Keywords

  • anandamide
  • bladder
  • cannabinoid
  • multiple sclerosis
  • receptors
  • urination

ASJC Scopus subject areas

  • Urology

Cite this

Gratzke, C., Streng, T., Park, A., Christ, G., Stief, C. G., Hedlund, P., & Andersson, K. E. (2009). Distribution and Function of Cannabinoid Receptors 1 and 2 in the Rat, Monkey and Human Bladder. Journal of Urology, 181(4), 1939-1948. https://doi.org/10.1016/j.juro.2008.11.079

Distribution and Function of Cannabinoid Receptors 1 and 2 in the Rat, Monkey and Human Bladder. / Gratzke, Christian; Streng, Tomi; Park, Anthony; Christ, George; Stief, Christian G.; Hedlund, Petter; Andersson, Karl Erik.

In: Journal of Urology, Vol. 181, No. 4, 04.2009, p. 1939-1948.

Research output: Contribution to journalArticle

Gratzke, C, Streng, T, Park, A, Christ, G, Stief, CG, Hedlund, P & Andersson, KE 2009, 'Distribution and Function of Cannabinoid Receptors 1 and 2 in the Rat, Monkey and Human Bladder', Journal of Urology, vol. 181, no. 4, pp. 1939-1948. https://doi.org/10.1016/j.juro.2008.11.079
Gratzke, Christian ; Streng, Tomi ; Park, Anthony ; Christ, George ; Stief, Christian G. ; Hedlund, Petter ; Andersson, Karl Erik. / Distribution and Function of Cannabinoid Receptors 1 and 2 in the Rat, Monkey and Human Bladder. In: Journal of Urology. 2009 ; Vol. 181, No. 4. pp. 1939-1948.
@article{e854425ddded46cbb78cb4addc5ec13b,
title = "Distribution and Function of Cannabinoid Receptors 1 and 2 in the Rat, Monkey and Human Bladder",
abstract = "Purpose: We investigated the distribution of cannabinoid receptor subtypes 1 and 2 in the detrusor of different species and studied the effects of cannabinoid receptor 1 and 2 agonists on bladder function. Materials and Methods: Cannabinoid receptor 1 and 2 expression was studied with Western blot and immunohistochemistry in rat, monkey and human detrusors. Co-staining was done for markers of sensory nerves using calcitonin gene-related peptide (Euro-Diagnostica, Malm{\"o}, Sweden) and transient receptor potential vanilloid 1, and for cholinergic nerves using VAChT (Santa Cruz Biotechnology, Santa Cruz, California). Actions of the endogenous cannabinoid receptor-1 and 2 agonist anandamide (Sigma{\circledR}), and the cannabinoid receptor 1 and 2 agonist CP55,940 (Sigma) on isolated detrusor and during cystometry in conscious rats were recorded. Results: Higher expression of cannabinoid receptor 2 but not cannabinoid receptor 1 was noted in the mucosa than in the detrusor. Compared to the detrusor larger amounts of cannabinoid receptor 2 containing nerves that also expressed transient receptor potential vanilloid 1 or calcitonin gene-related peptide were observed in the suburothelium. Nerve fibers containing cannabinoid receptor 2 and VAChT were located in the detrusor. Neither anandamide nor CP55,940 affected isolated detrusor carbachol (Sigma) contractions. Nerve contractions were enhanced by 10 μM anandamide and decreased by 10 μM CP55,940 (p",
keywords = "anandamide, bladder, cannabinoid, multiple sclerosis, receptors, urination",
author = "Christian Gratzke and Tomi Streng and Anthony Park and George Christ and Stief, {Christian G.} and Petter Hedlund and Andersson, {Karl Erik}",
year = "2009",
month = "4",
doi = "10.1016/j.juro.2008.11.079",
language = "English",
volume = "181",
pages = "1939--1948",
journal = "Journal of Urology",
issn = "0022-5347",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Distribution and Function of Cannabinoid Receptors 1 and 2 in the Rat, Monkey and Human Bladder

AU - Gratzke, Christian

AU - Streng, Tomi

AU - Park, Anthony

AU - Christ, George

AU - Stief, Christian G.

AU - Hedlund, Petter

AU - Andersson, Karl Erik

PY - 2009/4

Y1 - 2009/4

N2 - Purpose: We investigated the distribution of cannabinoid receptor subtypes 1 and 2 in the detrusor of different species and studied the effects of cannabinoid receptor 1 and 2 agonists on bladder function. Materials and Methods: Cannabinoid receptor 1 and 2 expression was studied with Western blot and immunohistochemistry in rat, monkey and human detrusors. Co-staining was done for markers of sensory nerves using calcitonin gene-related peptide (Euro-Diagnostica, Malmö, Sweden) and transient receptor potential vanilloid 1, and for cholinergic nerves using VAChT (Santa Cruz Biotechnology, Santa Cruz, California). Actions of the endogenous cannabinoid receptor-1 and 2 agonist anandamide (Sigma®), and the cannabinoid receptor 1 and 2 agonist CP55,940 (Sigma) on isolated detrusor and during cystometry in conscious rats were recorded. Results: Higher expression of cannabinoid receptor 2 but not cannabinoid receptor 1 was noted in the mucosa than in the detrusor. Compared to the detrusor larger amounts of cannabinoid receptor 2 containing nerves that also expressed transient receptor potential vanilloid 1 or calcitonin gene-related peptide were observed in the suburothelium. Nerve fibers containing cannabinoid receptor 2 and VAChT were located in the detrusor. Neither anandamide nor CP55,940 affected isolated detrusor carbachol (Sigma) contractions. Nerve contractions were enhanced by 10 μM anandamide and decreased by 10 μM CP55,940 (p

AB - Purpose: We investigated the distribution of cannabinoid receptor subtypes 1 and 2 in the detrusor of different species and studied the effects of cannabinoid receptor 1 and 2 agonists on bladder function. Materials and Methods: Cannabinoid receptor 1 and 2 expression was studied with Western blot and immunohistochemistry in rat, monkey and human detrusors. Co-staining was done for markers of sensory nerves using calcitonin gene-related peptide (Euro-Diagnostica, Malmö, Sweden) and transient receptor potential vanilloid 1, and for cholinergic nerves using VAChT (Santa Cruz Biotechnology, Santa Cruz, California). Actions of the endogenous cannabinoid receptor-1 and 2 agonist anandamide (Sigma®), and the cannabinoid receptor 1 and 2 agonist CP55,940 (Sigma) on isolated detrusor and during cystometry in conscious rats were recorded. Results: Higher expression of cannabinoid receptor 2 but not cannabinoid receptor 1 was noted in the mucosa than in the detrusor. Compared to the detrusor larger amounts of cannabinoid receptor 2 containing nerves that also expressed transient receptor potential vanilloid 1 or calcitonin gene-related peptide were observed in the suburothelium. Nerve fibers containing cannabinoid receptor 2 and VAChT were located in the detrusor. Neither anandamide nor CP55,940 affected isolated detrusor carbachol (Sigma) contractions. Nerve contractions were enhanced by 10 μM anandamide and decreased by 10 μM CP55,940 (p

KW - anandamide

KW - bladder

KW - cannabinoid

KW - multiple sclerosis

KW - receptors

KW - urination

UR - http://www.scopus.com/inward/record.url?scp=62049084948&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=62049084948&partnerID=8YFLogxK

U2 - 10.1016/j.juro.2008.11.079

DO - 10.1016/j.juro.2008.11.079

M3 - Article

VL - 181

SP - 1939

EP - 1948

JO - Journal of Urology

JF - Journal of Urology

SN - 0022-5347

IS - 4

ER -