Distribution of αlatrotoxin receptor in the rat brain by quantitative autoradiography: Comparison with the nerve terminal protein, synapsin I

A. Malgaroli, P. DeCamilui, J. Meldolesi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Tissue slice autoradiography was employed to reveal the brain distribution of the receptor for αlatrotoxin, the presynaptic neurotoxin of the black widow spider venom. The receptor distribution pattern was compared with that of a marker protein for nerve endings, synapsin I, a phosphoprotein known to be present within nerve terminals. The αlatrotoxin receptor and synapsin I were detected in gray matter-containing regions but their relative amounts were not constant. In the cerebral cortex and in the caudatum their distribution was similar, while in the hippocampus they were both abundant, but their distribution varied: synapsin I labeling was heavier in CA4 and CA3,αlatrotoxin receptor labeling in CA1 and dentate gyrus. A dissociation was also observed in the globus pallidus and in the lateral thalamic nuclear complex, where αlatrotoxin receptor labeling was very weak. The most striking dissociation occurred in the cerebellum, where the molecular layer was strongly labeled for synapsin I, but almost unlabeled for the αlatrotoxin receptor, which was more concentrated in the granular layer. Taken as a whole, the data appear compatible with a widespread localization of the αlatrotoxin receptor at synapses. However, they also suggest that either some nerve terminals are insensitive to αlatrotoxin, or the receptor for the toxin is not present at a similar concentration in all presynaptic plasma membranes.

Original languageEnglish
Pages (from-to)393-404
Number of pages12
JournalNeuroscience
Volume32
Issue number2
DOIs
Publication statusPublished - 1989

Fingerprint

Synapsins
Autoradiography
Brain
Proteins
Presynaptic Receptors
Urodela
Globus Pallidus
Nerve Endings
Phosphoproteins
Dentate Gyrus
Neurotoxins
Cerebral Cortex
Synapses
Cerebellum
Hippocampus
Cell Membrane

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Distribution of αlatrotoxin receptor in the rat brain by quantitative autoradiography : Comparison with the nerve terminal protein, synapsin I. / Malgaroli, A.; DeCamilui, P.; Meldolesi, J.

In: Neuroscience, Vol. 32, No. 2, 1989, p. 393-404.

Research output: Contribution to journalArticle

@article{f44723ce544641bd99d4cb7dcb89ee17,
title = "Distribution of αlatrotoxin receptor in the rat brain by quantitative autoradiography: Comparison with the nerve terminal protein, synapsin I",
abstract = "Tissue slice autoradiography was employed to reveal the brain distribution of the receptor for αlatrotoxin, the presynaptic neurotoxin of the black widow spider venom. The receptor distribution pattern was compared with that of a marker protein for nerve endings, synapsin I, a phosphoprotein known to be present within nerve terminals. The αlatrotoxin receptor and synapsin I were detected in gray matter-containing regions but their relative amounts were not constant. In the cerebral cortex and in the caudatum their distribution was similar, while in the hippocampus they were both abundant, but their distribution varied: synapsin I labeling was heavier in CA4 and CA3,αlatrotoxin receptor labeling in CA1 and dentate gyrus. A dissociation was also observed in the globus pallidus and in the lateral thalamic nuclear complex, where αlatrotoxin receptor labeling was very weak. The most striking dissociation occurred in the cerebellum, where the molecular layer was strongly labeled for synapsin I, but almost unlabeled for the αlatrotoxin receptor, which was more concentrated in the granular layer. Taken as a whole, the data appear compatible with a widespread localization of the αlatrotoxin receptor at synapses. However, they also suggest that either some nerve terminals are insensitive to αlatrotoxin, or the receptor for the toxin is not present at a similar concentration in all presynaptic plasma membranes.",
author = "A. Malgaroli and P. DeCamilui and J. Meldolesi",
year = "1989",
doi = "10.1016/0306-4522(89)90088-2",
language = "English",
volume = "32",
pages = "393--404",
journal = "Neuroscience",
issn = "0306-4522",
publisher = "Elsevier Limited",
number = "2",

}

TY - JOUR

T1 - Distribution of αlatrotoxin receptor in the rat brain by quantitative autoradiography

T2 - Comparison with the nerve terminal protein, synapsin I

AU - Malgaroli, A.

AU - DeCamilui, P.

AU - Meldolesi, J.

PY - 1989

Y1 - 1989

N2 - Tissue slice autoradiography was employed to reveal the brain distribution of the receptor for αlatrotoxin, the presynaptic neurotoxin of the black widow spider venom. The receptor distribution pattern was compared with that of a marker protein for nerve endings, synapsin I, a phosphoprotein known to be present within nerve terminals. The αlatrotoxin receptor and synapsin I were detected in gray matter-containing regions but their relative amounts were not constant. In the cerebral cortex and in the caudatum their distribution was similar, while in the hippocampus they were both abundant, but their distribution varied: synapsin I labeling was heavier in CA4 and CA3,αlatrotoxin receptor labeling in CA1 and dentate gyrus. A dissociation was also observed in the globus pallidus and in the lateral thalamic nuclear complex, where αlatrotoxin receptor labeling was very weak. The most striking dissociation occurred in the cerebellum, where the molecular layer was strongly labeled for synapsin I, but almost unlabeled for the αlatrotoxin receptor, which was more concentrated in the granular layer. Taken as a whole, the data appear compatible with a widespread localization of the αlatrotoxin receptor at synapses. However, they also suggest that either some nerve terminals are insensitive to αlatrotoxin, or the receptor for the toxin is not present at a similar concentration in all presynaptic plasma membranes.

AB - Tissue slice autoradiography was employed to reveal the brain distribution of the receptor for αlatrotoxin, the presynaptic neurotoxin of the black widow spider venom. The receptor distribution pattern was compared with that of a marker protein for nerve endings, synapsin I, a phosphoprotein known to be present within nerve terminals. The αlatrotoxin receptor and synapsin I were detected in gray matter-containing regions but their relative amounts were not constant. In the cerebral cortex and in the caudatum their distribution was similar, while in the hippocampus they were both abundant, but their distribution varied: synapsin I labeling was heavier in CA4 and CA3,αlatrotoxin receptor labeling in CA1 and dentate gyrus. A dissociation was also observed in the globus pallidus and in the lateral thalamic nuclear complex, where αlatrotoxin receptor labeling was very weak. The most striking dissociation occurred in the cerebellum, where the molecular layer was strongly labeled for synapsin I, but almost unlabeled for the αlatrotoxin receptor, which was more concentrated in the granular layer. Taken as a whole, the data appear compatible with a widespread localization of the αlatrotoxin receptor at synapses. However, they also suggest that either some nerve terminals are insensitive to αlatrotoxin, or the receptor for the toxin is not present at a similar concentration in all presynaptic plasma membranes.

UR - http://www.scopus.com/inward/record.url?scp=0024408086&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024408086&partnerID=8YFLogxK

U2 - 10.1016/0306-4522(89)90088-2

DO - 10.1016/0306-4522(89)90088-2

M3 - Article

C2 - 2555739

AN - SCOPUS:0024408086

VL - 32

SP - 393

EP - 404

JO - Neuroscience

JF - Neuroscience

SN - 0306-4522

IS - 2

ER -