Abstract
Analysis of saturation curves using selective labelling techniques in rabbit brain cortex showed that maximum binding capacities at μ-, δ- and κ-opioid binding sites were respectively 4.34, 1.68 and 5.43 pmol/g tissue and the binding affinities at the three sites were 2.98, 0.52 and 0.091 nM. The binding of single concentrations of tritiated ligands (1 x k(D) value in the cortex) was determined at μ-, δ- and κ-sites in seven rabbit brain regions. The highest content of μ-sites was in the striatum, followed by the diencephalon, mesencephalon, cerebellum and cortex; the lowest content was in the pons-medulla and the hippocampus. All regions had a content in δ-sites similar to the cortex, which had fewer δ-sites than μ- and κ-sites. The content in κ-sites was highest in the diencephalon and lowest in the mesencephalon, cerebellum hippocampus and pons-medulla.
| Original language | English |
|---|---|
| Pages (from-to) | 1-4 |
| Number of pages | 4 |
| Journal | Journal of Endocrinological Investigation |
| Volume | 12 |
| Issue number | 10 SUPPL. 4 |
| Publication status | Published - 1989 |
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ASJC Scopus subject areas
- Endocrinology
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Distribution of μ-, δ- and κ-opioid binding sites in membrane suspensions from rabbit brain regions. / Tavani, A.; Petrillo, P.; La Regina, A.; Sbacchi, M.
In: Journal of Endocrinological Investigation, Vol. 12, No. 10 SUPPL. 4, 1989, p. 1-4.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Distribution of μ-, δ- and κ-opioid binding sites in membrane suspensions from rabbit brain regions
AU - Tavani, A.
AU - Petrillo, P.
AU - La Regina, A.
AU - Sbacchi, M.
PY - 1989
Y1 - 1989
N2 - Analysis of saturation curves using selective labelling techniques in rabbit brain cortex showed that maximum binding capacities at μ-, δ- and κ-opioid binding sites were respectively 4.34, 1.68 and 5.43 pmol/g tissue and the binding affinities at the three sites were 2.98, 0.52 and 0.091 nM. The binding of single concentrations of tritiated ligands (1 x k(D) value in the cortex) was determined at μ-, δ- and κ-sites in seven rabbit brain regions. The highest content of μ-sites was in the striatum, followed by the diencephalon, mesencephalon, cerebellum and cortex; the lowest content was in the pons-medulla and the hippocampus. All regions had a content in δ-sites similar to the cortex, which had fewer δ-sites than μ- and κ-sites. The content in κ-sites was highest in the diencephalon and lowest in the mesencephalon, cerebellum hippocampus and pons-medulla.
AB - Analysis of saturation curves using selective labelling techniques in rabbit brain cortex showed that maximum binding capacities at μ-, δ- and κ-opioid binding sites were respectively 4.34, 1.68 and 5.43 pmol/g tissue and the binding affinities at the three sites were 2.98, 0.52 and 0.091 nM. The binding of single concentrations of tritiated ligands (1 x k(D) value in the cortex) was determined at μ-, δ- and κ-sites in seven rabbit brain regions. The highest content of μ-sites was in the striatum, followed by the diencephalon, mesencephalon, cerebellum and cortex; the lowest content was in the pons-medulla and the hippocampus. All regions had a content in δ-sites similar to the cortex, which had fewer δ-sites than μ- and κ-sites. The content in κ-sites was highest in the diencephalon and lowest in the mesencephalon, cerebellum hippocampus and pons-medulla.
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M3 - Article
VL - 12
SP - 1
EP - 4
JO - Journal of Endocrinological Investigation
JF - Journal of Endocrinological Investigation
SN - 0391-4097
IS - 10 SUPPL. 4
ER -