Distribution of acid alpha-naphthyl acetate esterase among human T lymphocyte subsets

Andrea Facchini, Adriana Rita Mariani, Stefano Papa, Erminia Mariani, Francesco Antonio Manzoli

Research output: Contribution to journalArticlepeer-review

Abstract

Human T lymphocyte subsets, identified by means of OKT3, 4 and 8 monoclonal antibodies, were isolated by a fluorescence activated cell sorter (FACS IV) and analyzed for distribution of alpha-naphthyl acetate esterase (ANAE) activity. As compared to OKT8+ lymphocytes a higher proportion of OKT4+ lymphocytes was ANAE-positive exibiting a spot or dot-like pattern in the cytoplasm. OKT8 and 4 positive subsets showed a similar ANAE distribution in diffuse granular form. Although OKT4 and OKT8 populations presented a different ANAE dot-like reactivity, this marker did not allow as clear a distinction between them as that reported for TG and TM lymphocytes.

Original languageEnglish
Pages (from-to)207-210
Number of pages4
JournalImmunology Letters
Volume8
Issue number4
DOIs
Publication statusPublished - 1984

Keywords

  • alpha-naphthyl acetate esterase
  • flow cytometry
  • OKT monoclonal antibodies

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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