Distribution of centromeric proteins and PARP-1 during mitosis and apoptosis

Federica Perdoni, Maria Grazia Bottone, Cristiana Soldani, Paola Veneroni, Claudia Alpini, Carlo Pellicciari, Anna Ivana Scovassi

Research output: Chapter in Book/Report/Conference proceedingConference contribution


A large complex of proteins, called CENPs, are associated with centromeric DNA. Some of them exhibit a cell cycle-related expression (e.g., CENP-E and -F) and are required for the transition from interphase to mitosis, whereas constitutive proteins (e.g., CENP-A, -B, -C, -G, and -H) reside permanently at the centromere and are essential for the correct kinetochore assembly. Poly(ADP-ribose) polymerase-1 (PARP-1), which plays an active role in many basic processes, was described as a possible regulator of CENPs. By multicolor immunofluorescence we therefore analyzed the distribution of PARP-1 and its interaction with CENP-B, -E, and -F during mitosis and apoptosis.

Original languageEnglish
Title of host publicationAnnals of the New York Academy of Sciences
Number of pages6
Publication statusPublished - Aug 2009

Publication series

NameAnnals of the New York Academy of Sciences
ISSN (Print)00778923
ISSN (Electronic)17496632


  • Apoptosis
  • CENPs
  • Fluorescence microscopy
  • Immunocytochemistry
  • Mitosis
  • PARP-1

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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