Distribution of estrogen receptor subtypes, expression of their variant forms, and clinicopathological characteristics of human colorectal cancer

Aldo Cavallini, Caterina Messa, Maria Pricci, Maria Lucia Caruso, Michele Barone, Alfredo Di Leo

Research output: Contribution to journalArticle

Abstract

We examined the expression of estrogen receptor (ER) messenger RNAs (ER-α, ER-β, and ER-β isoforms) in colorectal tumor samples and corresponding normal mucosa, paying particular attention exons 3 and 5 of both ER mRNA subtypes that likely suffer deletions, and may encode proteins that have lost either DNA- or ligand-binding moieties. Then we correlated these findings with the clinicopathological properties of the tumors. Our results demonstrated that in all patients the two ER subtype mRNAs were coexpressed in wild-type form. In 10% of the patients the ER-α mRNA was also present as an exon-5-deleted form that encoded any aberrant protein. Immunohistochemical analysis revealed that the ER-β protein was present in tumor stroma, but not in infiltrating lymphocytes. ER-β1 and ER-β2, isoforms of ER-β, were up-regulated in malignant tissues, whereas the ER-β5 isoform, was found to be equally expressed; at very low levels, in the two tissue compartments. No correlations between ER levels and clinicopathological parameters were found. This suggests that the ER-β mRNA levels are independent of the tumor characteristics.

Original languageEnglish
Pages (from-to)2720-2728
Number of pages9
JournalDigestive Diseases and Sciences
Volume47
Issue number12
DOIs
Publication statusPublished - Dec 1 2002

Keywords

  • Colorectal adenocarcinoma
  • Estrogen receptor alpha
  • Estrogen receptor beta
  • Estrogen receptor variant form

ASJC Scopus subject areas

  • Gastroenterology

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