Distribution of GABA(B) receptor protein in somatosensory cortex and thalamus of adult rats and during postnatal development

Alessandra Princivalle, Maria Cristina Regondi, Carolina Frassoni, Norman G. Bowery, Roberto Spreafico

Research output: Contribution to journalArticle

Abstract

In the present study we report the immunolocalisation of γ-aminobutyric acid (GABA)(B) receptors within the cerebral somatosensory cortex (S1) and thalamus of adult and young (1-22 postnatal days) rats. The antibody used recognises a peptide in the carboxy-terminal domain and therefore did not distinguish between the different isoforms GABA(B)1a or GABA(B)1b. The results showed that GABA(B) receptor protein was widely distributed in the brain of both adult and young rats, with different degrees of labelling in separate cerebral nuclei. Antibody labelling was localised both on cells and the neuropil. In the cerebral cortex of adult animals the highest immunolabelling was evident in layers V and VIb, although immunoreactivity was also present in the superficial layers. The strongest signal was evident in the medial habenula.The thalamus showed labelling in the reticular, ventrobasal and geniculate nuclei. In the first postnatal days GABA(B) expression was evident in the cortical cells of layer V, VIb and in the cortical plate. The pattern of labelling in the cerebral cortex of young rats became indistinguishable from that of adult rats by day 12. In the thalamus, the main difference compared to the adult pattern was observed in the mediodorsal nucleus which, in early development, showed a high immunosignal, however, by postnatal day 22 the immunoreactivity decreased with only some scattered cells labelled in the adult brain. Copyright (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)397-405
Number of pages9
JournalBrain Research Bulletin
Volume52
Issue number5
DOIs
Publication statusPublished - Jul 15 2000

Keywords

  • Cortical layers
  • Diencephalon
  • GABA
  • Immunolocalisation
  • Maturation
  • Metabotropic receptors
  • Thalamic mediodorsal nucleus

ASJC Scopus subject areas

  • Neuroscience(all)

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