Distribution of gephyrin transcripts in the adult and developing rat brain

J. Kirsch, M. L. Malosio, I. Wolters, H. Betz

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

The peripheral membrane protein gephyrin copurifies with the inhibitory glycine receptor of mammalian spinal cord. It binds with high affinity to polymerized tubulin and has been implicated in the anchoring of the glycine receptor to cytoskeletal elements. Recently, cDNA cloning has identified variants of the gephyrin mRNA, which originate from alternative splicing of four exonic regions (cassettes 1-4). In this study, the expression patterns of gephyrin splice variants were determined in the adult and developing rat brain by in situ hybridization with synthetic oligonucleotide probes. Gephyrin transcripts were detected throughout the brain and spinal cord, with mRNAs containing cassette 2 (C2 transcripts) being predominant in adult animals. C3 and C4 transcripts were seen in cerebellar granule cells and in the dentate gyrus, whereas a Cl probe did not produce detectable hybridization signals. During development, C2 and C3 mRNAs were found in most brain regions. Generally, the spatial and temporal distribution of gephyrin transcripts is similar to that of the glycine receptor β subunit mRNA reported previously.

Original languageEnglish
Pages (from-to)1109-1117
Number of pages9
JournalEuropean Journal of Neuroscience
Volume5
Issue number9
Publication statusPublished - 1993

Fingerprint

Glycine Receptors
Brain
Messenger RNA
Spinal Cord
Oligonucleotide Probes
Dentate Gyrus
Alternative Splicing
Tubulin
In Situ Hybridization
Organism Cloning
Membrane Proteins
Complementary DNA
gephyrin

Keywords

  • Glycine receptor
  • In situ hybridization
  • Receptor-associated protein
  • Tubulin binding protein

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Distribution of gephyrin transcripts in the adult and developing rat brain. / Kirsch, J.; Malosio, M. L.; Wolters, I.; Betz, H.

In: European Journal of Neuroscience, Vol. 5, No. 9, 1993, p. 1109-1117.

Research output: Contribution to journalArticle

Kirsch, J. ; Malosio, M. L. ; Wolters, I. ; Betz, H. / Distribution of gephyrin transcripts in the adult and developing rat brain. In: European Journal of Neuroscience. 1993 ; Vol. 5, No. 9. pp. 1109-1117.
@article{faf269088fca4bf88f6276037cd423d5,
title = "Distribution of gephyrin transcripts in the adult and developing rat brain",
abstract = "The peripheral membrane protein gephyrin copurifies with the inhibitory glycine receptor of mammalian spinal cord. It binds with high affinity to polymerized tubulin and has been implicated in the anchoring of the glycine receptor to cytoskeletal elements. Recently, cDNA cloning has identified variants of the gephyrin mRNA, which originate from alternative splicing of four exonic regions (cassettes 1-4). In this study, the expression patterns of gephyrin splice variants were determined in the adult and developing rat brain by in situ hybridization with synthetic oligonucleotide probes. Gephyrin transcripts were detected throughout the brain and spinal cord, with mRNAs containing cassette 2 (C2 transcripts) being predominant in adult animals. C3 and C4 transcripts were seen in cerebellar granule cells and in the dentate gyrus, whereas a Cl probe did not produce detectable hybridization signals. During development, C2 and C3 mRNAs were found in most brain regions. Generally, the spatial and temporal distribution of gephyrin transcripts is similar to that of the glycine receptor β subunit mRNA reported previously.",
keywords = "Glycine receptor, In situ hybridization, Receptor-associated protein, Tubulin binding protein",
author = "J. Kirsch and Malosio, {M. L.} and I. Wolters and H. Betz",
year = "1993",
language = "English",
volume = "5",
pages = "1109--1117",
journal = "European Journal of Neuroscience",
issn = "0953-816X",
publisher = "Wiley-Blackwell",
number = "9",

}

TY - JOUR

T1 - Distribution of gephyrin transcripts in the adult and developing rat brain

AU - Kirsch, J.

AU - Malosio, M. L.

AU - Wolters, I.

AU - Betz, H.

PY - 1993

Y1 - 1993

N2 - The peripheral membrane protein gephyrin copurifies with the inhibitory glycine receptor of mammalian spinal cord. It binds with high affinity to polymerized tubulin and has been implicated in the anchoring of the glycine receptor to cytoskeletal elements. Recently, cDNA cloning has identified variants of the gephyrin mRNA, which originate from alternative splicing of four exonic regions (cassettes 1-4). In this study, the expression patterns of gephyrin splice variants were determined in the adult and developing rat brain by in situ hybridization with synthetic oligonucleotide probes. Gephyrin transcripts were detected throughout the brain and spinal cord, with mRNAs containing cassette 2 (C2 transcripts) being predominant in adult animals. C3 and C4 transcripts were seen in cerebellar granule cells and in the dentate gyrus, whereas a Cl probe did not produce detectable hybridization signals. During development, C2 and C3 mRNAs were found in most brain regions. Generally, the spatial and temporal distribution of gephyrin transcripts is similar to that of the glycine receptor β subunit mRNA reported previously.

AB - The peripheral membrane protein gephyrin copurifies with the inhibitory glycine receptor of mammalian spinal cord. It binds with high affinity to polymerized tubulin and has been implicated in the anchoring of the glycine receptor to cytoskeletal elements. Recently, cDNA cloning has identified variants of the gephyrin mRNA, which originate from alternative splicing of four exonic regions (cassettes 1-4). In this study, the expression patterns of gephyrin splice variants were determined in the adult and developing rat brain by in situ hybridization with synthetic oligonucleotide probes. Gephyrin transcripts were detected throughout the brain and spinal cord, with mRNAs containing cassette 2 (C2 transcripts) being predominant in adult animals. C3 and C4 transcripts were seen in cerebellar granule cells and in the dentate gyrus, whereas a Cl probe did not produce detectable hybridization signals. During development, C2 and C3 mRNAs were found in most brain regions. Generally, the spatial and temporal distribution of gephyrin transcripts is similar to that of the glycine receptor β subunit mRNA reported previously.

KW - Glycine receptor

KW - In situ hybridization

KW - Receptor-associated protein

KW - Tubulin binding protein

UR - http://www.scopus.com/inward/record.url?scp=0027171989&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027171989&partnerID=8YFLogxK

M3 - Article

C2 - 8281317

AN - SCOPUS:0027171989

VL - 5

SP - 1109

EP - 1117

JO - European Journal of Neuroscience

JF - European Journal of Neuroscience

SN - 0953-816X

IS - 9

ER -