Distribution of GFRA1-expressing spermatogonia in adult mouse testis

Margherita Grasso, Andrea Fuso, Lisa Dovere, Dirk G. De Rooij, Mario Stefanini, Carla Boitani, Elena Vicini

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

In mice and other mammals, spermatogenesis is maintained by spermatogonial stem cells (SSCs), a cell population belonging to undifferentiated type A spermatogonia. In the accepted model of SSC self-renewal, Asingle (As) spermatogonia are the stem cells, whereas paired (Apaired (Apr)) and chained (Aaligned (Aal)) undifferentiated spermatogonia are committed to differentiation. This model has been recently challenged by evidence that As and chained (Apr and Aal), undifferentiated spermatogonia are heterogeneous in terms of gene expression and function. The expression profile of several markers, such as GFRA1 (the GDNF co-receptor), is heterogeneous among As, Apr and Aal spermatogonia. In this study, we have analysed and quantified the distribution of GFRA1-expressing cells within the different stages of the seminiferous epithelial cycle. We show that in all stages, GFRA1 + chained spermatogonia (Apr to Aal) are more numerous than GFRA1+ As spermatogonia. Numbers of chained GFRA1+ spermatogonia are sharply reduced in stages VII-VIII when Aal differentiate into A1 spermatogonia. GFRA1 expression is regulated by GDNF and in cultures of isolated seminiferous tubules, we found that GDNF expression and secretion by Sertoli cells is stage-dependent, being maximal in stages II-VI and decreasing thereafter. Using qRT-PCR analysis, we found that GDNF regulates the expression of genes such as Tex14, Sohlh1 and Kit (c-Kit) known to be involved in spermatogonial differentiation. Expression of Kit was upregulated by GDNF in a stage-specific manner. Our data indicate that GDNF, besides its crucial role in the self-renewal of stem cells also functions in the differentiation of chained undifferentiated spermatogonia.

Original languageEnglish
Pages (from-to)325-332
Number of pages8
JournalReproduction
Volume143
Issue number3
DOIs
Publication statusPublished - Mar 2012

Fingerprint

Spermatogonia
Testis
Glial Cell Line-Derived Neurotrophic Factor
Glial Cell Line-Derived Neurotrophic Factor Receptors
Stem Cells
Gene Expression
Seminiferous Tubules
Sertoli Cells
Spermatogenesis
Mammals
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine
  • Embryology
  • Endocrinology
  • Cell Biology

Cite this

Grasso, M., Fuso, A., Dovere, L., De Rooij, D. G., Stefanini, M., Boitani, C., & Vicini, E. (2012). Distribution of GFRA1-expressing spermatogonia in adult mouse testis. Reproduction, 143(3), 325-332. https://doi.org/10.1530/REP-11-0385

Distribution of GFRA1-expressing spermatogonia in adult mouse testis. / Grasso, Margherita; Fuso, Andrea; Dovere, Lisa; De Rooij, Dirk G.; Stefanini, Mario; Boitani, Carla; Vicini, Elena.

In: Reproduction, Vol. 143, No. 3, 03.2012, p. 325-332.

Research output: Contribution to journalArticle

Grasso, M, Fuso, A, Dovere, L, De Rooij, DG, Stefanini, M, Boitani, C & Vicini, E 2012, 'Distribution of GFRA1-expressing spermatogonia in adult mouse testis', Reproduction, vol. 143, no. 3, pp. 325-332. https://doi.org/10.1530/REP-11-0385
Grasso M, Fuso A, Dovere L, De Rooij DG, Stefanini M, Boitani C et al. Distribution of GFRA1-expressing spermatogonia in adult mouse testis. Reproduction. 2012 Mar;143(3):325-332. https://doi.org/10.1530/REP-11-0385
Grasso, Margherita ; Fuso, Andrea ; Dovere, Lisa ; De Rooij, Dirk G. ; Stefanini, Mario ; Boitani, Carla ; Vicini, Elena. / Distribution of GFRA1-expressing spermatogonia in adult mouse testis. In: Reproduction. 2012 ; Vol. 143, No. 3. pp. 325-332.
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