Murine peritoneal macrophages (pM∅) elicited in vivo by intraperitoneal (IP) inoculation of various agents were tested for their homing/distribution patterns after intravenous (IV) adoptive transfer to syngeneic C57BL/6 recipients. Resident pM∅ (RpM∅) obtained from normal mice and pM∅ elicited by proteose peptone (PpM∅) or thioglycollate broth (TpM∅) exhibited similar homing paterns following IV transfer. After initial arrest in the lungs, these cells rapidly disseminated to liver and spleen, with minimal or no detectable migration to peripheral lymph nodes, intestine, peritoneum, kidney, heart, or retention in the blood. The pattern of results reflected the properties of pM∅ themselves, since highly enriched pM∅ populations obtained by treatment of crude peritoneal exudate cells with anti-Thy 1.2 + C, or by fractionation on Percoll density gradients, gave similar results. The distribution of pM∅ elicited by Brewer's thioglycollate medium (BTpM∅) was markedly different from other pM∅ tested. BTpM∅ homed rapidly to the lungs and many remained localized there for at least 72 hr with very little migration to the spleen. The distribution of PpM∅ could be altered by activation of these cells in vivo through the IP injection of the pyran copolymer, MVE-2, prior to adoptive IV transfer. Activated PpM∅ contained a population of highly differentiated, low density pM∅, separable on density gradients, which arrested in the lungs for appreciably longer periods of time than did PpM∅. These cells exhibited reduced ability for migration to the spleen. Macrophage-like (M∅-like) cell lines did not exhibit migration capability, but rather were rapidly cleared from the circulation in a manner similar to other types of tumor cells.
|Number of pages||17|
|Journal||RES Journal of the Reticuloendothelial Society|
|Publication status||Published - 1983|
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