Distribution pattern of hepatitis C Virus genotypes and correlation with viral load and risk factors in chronic positive patients

Arnolfo Petruzziello, Nicola Coppola, Giovanna Loquercio, Samantha Marigliano, Margherita Giordano, Rosa Azzaro, Anna Maria Diodato, Vincenzo Iervolino, Gaetano Di Costanzo, Catia Addolorata Di Macchia, Tommaso Di Meo, Laurenza Paradiso, Rosario Ferro, Pasquale Giuliano, Ferdinando Russo, Giuseppe Pasquale, Carmela Cacciapuoti

Research output: Contribution to journalArticle

Abstract

Objective: Hepatitis C virus (HCV) has emerged as a leading cause of chronic hepatitis, liver cirrhosis and hepatocellular carcinoma worldwide. The purpose of this study was to describe the distribution pattern of HCV genotypes in chronic hepatitis patients in the Campania region of southern Italy and estimate their association with risk factors and viral load.

Materials and Methods: 404 consecutive HCV ribonucleic acid-positive patients were included in the study. HCV genotyping was carried out by the HCV line probe assay test and viral load estimation by the TaqMan real-time PCR system.

Results: The predominant genotype was 1 (63.6%), followed by genotype 2 (29.4%), 3 (6.2%) and 4 (0.8%). Subtype 1b was more frequent in females than in males. Conversely, genotype 3 was more frequent in males. No significant difference was observed in age distribution of HCV genotypes. Surgery and dental therapy were the most frequent risk factors for genotype 1 and intravenous drug abuse and tattooing for genotype 3. Patients with genotype 1 more frequently showed high HCV viral load when compared to those with genotypes 2 and 3.

Conclusion: The present study revealed that HCV genotypes 1 and 2 accounted for over 95% of all HCV infections in the Campania region, and genotype 1 was more frequently associated with a higher viral load when compared to genotypes 2 and 3.

Original languageEnglish
Pages (from-to)311-318
Number of pages8
JournalIntervirology
Volume57
Issue number6
DOIs
Publication statusPublished - Dec 17 2014

Keywords

  • Chronic hepatitis
  • HCV genotyping
  • Hepatocellular carcinoma
  • Infected carriers
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Medicine(all)

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