TY - JOUR
T1 - Distribution pattern of hepatitis C Virus genotypes and correlation with viral load and risk factors in chronic positive patients
AU - Petruzziello, Arnolfo
AU - Coppola, Nicola
AU - Loquercio, Giovanna
AU - Marigliano, Samantha
AU - Giordano, Margherita
AU - Azzaro, Rosa
AU - Diodato, Anna Maria
AU - Iervolino, Vincenzo
AU - Di Costanzo, Gaetano
AU - Di Macchia, Catia Addolorata
AU - Di Meo, Tommaso
AU - Paradiso, Laurenza
AU - Ferro, Rosario
AU - Giuliano, Pasquale
AU - Russo, Ferdinando
AU - Pasquale, Giuseppe
AU - Cacciapuoti, Carmela
PY - 2014/12/17
Y1 - 2014/12/17
N2 - Objective: Hepatitis C virus (HCV) has emerged as a leading cause of chronic hepatitis, liver cirrhosis and hepatocellular carcinoma worldwide. The purpose of this study was to describe the distribution pattern of HCV genotypes in chronic hepatitis patients in the Campania region of southern Italy and estimate their association with risk factors and viral load.Materials and Methods: 404 consecutive HCV ribonucleic acid-positive patients were included in the study. HCV genotyping was carried out by the HCV line probe assay test and viral load estimation by the TaqMan real-time PCR system.Results: The predominant genotype was 1 (63.6%), followed by genotype 2 (29.4%), 3 (6.2%) and 4 (0.8%). Subtype 1b was more frequent in females than in males. Conversely, genotype 3 was more frequent in males. No significant difference was observed in age distribution of HCV genotypes. Surgery and dental therapy were the most frequent risk factors for genotype 1 and intravenous drug abuse and tattooing for genotype 3. Patients with genotype 1 more frequently showed high HCV viral load when compared to those with genotypes 2 and 3.Conclusion: The present study revealed that HCV genotypes 1 and 2 accounted for over 95% of all HCV infections in the Campania region, and genotype 1 was more frequently associated with a higher viral load when compared to genotypes 2 and 3.
AB - Objective: Hepatitis C virus (HCV) has emerged as a leading cause of chronic hepatitis, liver cirrhosis and hepatocellular carcinoma worldwide. The purpose of this study was to describe the distribution pattern of HCV genotypes in chronic hepatitis patients in the Campania region of southern Italy and estimate their association with risk factors and viral load.Materials and Methods: 404 consecutive HCV ribonucleic acid-positive patients were included in the study. HCV genotyping was carried out by the HCV line probe assay test and viral load estimation by the TaqMan real-time PCR system.Results: The predominant genotype was 1 (63.6%), followed by genotype 2 (29.4%), 3 (6.2%) and 4 (0.8%). Subtype 1b was more frequent in females than in males. Conversely, genotype 3 was more frequent in males. No significant difference was observed in age distribution of HCV genotypes. Surgery and dental therapy were the most frequent risk factors for genotype 1 and intravenous drug abuse and tattooing for genotype 3. Patients with genotype 1 more frequently showed high HCV viral load when compared to those with genotypes 2 and 3.Conclusion: The present study revealed that HCV genotypes 1 and 2 accounted for over 95% of all HCV infections in the Campania region, and genotype 1 was more frequently associated with a higher viral load when compared to genotypes 2 and 3.
KW - Chronic hepatitis
KW - HCV genotyping
KW - Hepatocellular carcinoma
KW - Infected carriers
KW - Polymerase chain reaction
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U2 - 10.1159/000363386
DO - 10.1159/000363386
M3 - Article
C2 - 25170801
AN - SCOPUS:84906596715
VL - 57
SP - 311
EP - 318
JO - Intervirology
JF - Intervirology
SN - 0300-5526
IS - 6
ER -