TY - JOUR
T1 - Distributional changes in gene-specific methylation associated with temperature
AU - Bind, Marie Abele C
AU - Coull, Brent A.
AU - Baccarelli, Andrea
AU - Tarantini, Letizia
AU - Cantone, Laura
AU - Vokonas, Pantel
AU - Schwartz, Joel
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Temperature has been related to mean differences in DNA methylation. However, heterogeneity in these associations may exist across the distribution of methylation outcomes. This study examined whether the association between three-week averaged of temperature and methylation differs across quantiles of the methylation distributions in nine candidate genes. We measured gene-specific blood methylation repeatedly in 777 elderly men participating in the Normative Aging Study (1999-2010). We fit quantile regressions for longitudinal data to investigate whether the associations of temperature on methylation (expressed as %5mC) varied across the distribution of the methylation outcomes. We observed heterogeneity in the associations of temperature across percentiles of methylation in F3, TLR-2, CRAT, iNOS, and ICAM-1 genes. For instance, an increase in three-week temperature exposure was associated with a longer left-tail of the F3 methylation distribution. A 5 °C increase in temperature was associated with a 0.15%5mC (95% confidence interval (CI): -0.27,-0.04) decrease on the 20th quantile of F3 methylation, but was not significantly related to the 80th quantile of this distribution (Estimate:0.06%5mC, 95%CI: -0.22, 0.35). Individuals with low values of F3, TLR-2, CRAT, and iNOS methylation, as well as a high value of ICAM-1 methylation, may be more susceptible to temperature effects on systemic inflammation.
AB - Temperature has been related to mean differences in DNA methylation. However, heterogeneity in these associations may exist across the distribution of methylation outcomes. This study examined whether the association between three-week averaged of temperature and methylation differs across quantiles of the methylation distributions in nine candidate genes. We measured gene-specific blood methylation repeatedly in 777 elderly men participating in the Normative Aging Study (1999-2010). We fit quantile regressions for longitudinal data to investigate whether the associations of temperature on methylation (expressed as %5mC) varied across the distribution of the methylation outcomes. We observed heterogeneity in the associations of temperature across percentiles of methylation in F3, TLR-2, CRAT, iNOS, and ICAM-1 genes. For instance, an increase in three-week temperature exposure was associated with a longer left-tail of the F3 methylation distribution. A 5 °C increase in temperature was associated with a 0.15%5mC (95% confidence interval (CI): -0.27,-0.04) decrease on the 20th quantile of F3 methylation, but was not significantly related to the 80th quantile of this distribution (Estimate:0.06%5mC, 95%CI: -0.22, 0.35). Individuals with low values of F3, TLR-2, CRAT, and iNOS methylation, as well as a high value of ICAM-1 methylation, may be more susceptible to temperature effects on systemic inflammation.
KW - DNA methylation
KW - Quantile regression
KW - Susceptibility
KW - Temperature
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U2 - 10.1016/j.envres.2016.05.034
DO - 10.1016/j.envres.2016.05.034
M3 - Article
AN - SCOPUS:84973129403
VL - 150
SP - 38
EP - 46
JO - Environmental Research
JF - Environmental Research
SN - 0013-9351
ER -