Disturbances in purinergic [Ca2+]i signaling pathways in a transformed rat thyroid cell line

Maria Giovanna Elia, Antonella Muscella, Simona Greco, Sebastiano Vilella, Carlo Storelli, Santo Marsigliante

Research output: Contribution to journalArticle

Abstract

It was previously shown that in rat thyroid PC-C13 cell line, a purinergic P2Y receptor increases the concentration of free cytosolic Ca2+ ([Ca2+]i) via phospholipase C activation. We here studied whether in a transformed cell line (PC-E1Araf) derived from parental PC-C13 cells, ATP is still able to transduce the [Ca2+]i-based intracellular signal. We demonstrate the expression of mRNA for P2Y2 in both PC-C13 and PC-E1Araf cells; mRNAs for P2Y1, P2Y4, P2Y6 and P2Y11 were absent. In both cell lines activation of P2Y2 receptor provokes a transient increase in [Ca2+]i followed by a lower sustained phase persisting for over 5 min in PC-C13 and only 1.5 min in PC-E1Araf cells. In both cell lines the [Ca2+]i reached a plateau level significantly higher than the basal [Ca2+]i level persisting for over 10 min. Removal of extracellular Ca2+ reduced the initial transient response to ATP in PC-C13, but not in PC-E1Araf cells, and completely abolished the plateau phase in both cell lines. In the presence of extracellular Ca2+ thapsigargin (TG) caused a rise in [Ca2+]i significantly higher in PC-C13 than transformed PC-E1Araf cells, while in Ca2+-free medium the effect of TG was similar in both cell lines. The capacitative Ca2+-entry in PC-C13 resulted significantly higher than in PC-E1Araf cells. Further studies were performed in order to investigate whether the different effects of ATP on [Ca2+]i was due to variation in divalent cation plasma membrane permeability. PC-E1Araf cells showed a much lower permeability to Ca2+, Ba2+, Sr2+, Mn2+, and Co2+ that may be responsible for the differences in purinergic Ca2+ signaling pathway with respect to parental PC-C13 cells.

Original languageEnglish
Pages (from-to)59-68
Number of pages10
JournalCell Calcium
Volume33
Issue number1
DOIs
Publication statusPublished - Jan 1 2003

Keywords

  • ATP
  • Ca
  • P2Y
  • PC-C13
  • PC-E1 Araf
  • Thyroid

ASJC Scopus subject areas

  • Cell Biology
  • Endocrinology

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