Disulfide-linked surface molecules of monoclonal antigen-specific suppressor T cells: Evidence for T cell receptor structures

D. Ballinari, C. Castelli, C. Traversari, M. A. Pierotti, G. Parmiani, G. Palmieri, P. Ricciardi-Castagnoli, L. Adorini

Research output: Contribution to journalArticle

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Abstract

By two-dimensional polyacrylamide gel electrophoresis analysis under nonreducing/reducing conditions, five proteins with interchain disulfide bridges are revealed on the surface of the suppressor T cell lymphoma line LH8-105 obtained by radiation leukemia virus-induced transformation of hen egg-white lysozyme-specific suppressor T lymphocytes. Two disulfide-linked surface proteins expressed by LH8-105 cells have been positively identified by immunoprecipitation with specific antisera. The major labeled membrane protein of LH8-105 cells is the murine leukemia virus env glycoprotein gp70. The second disulfide-linked molecule identified on LH8-105 cells has a molecular mass of 84 kDa under nonreducing conditions and 42 kDa after reduction, and is immunoprecipitated by an antiserum which recognizes the T cell receptor for antigen. A disulfide-linked molecule of a similar molecular mass is also immunoprecipitated from surface-labeled LH8-105 cells by a rabbit antiserum directed against a synthetic peptide predicted from the nucleotide sequence of a cDNA clone encoding the β chain constant region of a helper T cell hybridoma. Therefore, a dimeric structure comparable to the T cell receptor expressed by cytotoxic and helper T cells is present on the cell surface of these monoclonal antigen-specific suppressor T cells.

Original languageEnglish
Pages (from-to)855-860
Number of pages6
JournalEuropean Journal of Immunology
Volume15
Issue number8
DOIs
Publication statusPublished - 1985

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T-Cell Antigen Receptor
Disulfides
T-Lymphocytes
Antigens
Immune Sera
Helper-Inducer T-Lymphocytes
Radiation Leukemia Virus
Membrane Proteins
env Gene Products
Murine Leukemia Viruses
Egg White
T-Cell Lymphoma
Electrophoresis, Gel, Two-Dimensional
Hybridomas
Surface Antigens
Immunoprecipitation
Complementary DNA
Clone Cells
Rabbits
Cell Line

ASJC Scopus subject areas

  • Immunology

Cite this

Disulfide-linked surface molecules of monoclonal antigen-specific suppressor T cells : Evidence for T cell receptor structures. / Ballinari, D.; Castelli, C.; Traversari, C.; Pierotti, M. A.; Parmiani, G.; Palmieri, G.; Ricciardi-Castagnoli, P.; Adorini, L.

In: European Journal of Immunology, Vol. 15, No. 8, 1985, p. 855-860.

Research output: Contribution to journalArticle

Ballinari, D, Castelli, C, Traversari, C, Pierotti, MA, Parmiani, G, Palmieri, G, Ricciardi-Castagnoli, P & Adorini, L 1985, 'Disulfide-linked surface molecules of monoclonal antigen-specific suppressor T cells: Evidence for T cell receptor structures', European Journal of Immunology, vol. 15, no. 8, pp. 855-860. https://doi.org/10.1002/eji.1830150822
Ballinari, D. ; Castelli, C. ; Traversari, C. ; Pierotti, M. A. ; Parmiani, G. ; Palmieri, G. ; Ricciardi-Castagnoli, P. ; Adorini, L. / Disulfide-linked surface molecules of monoclonal antigen-specific suppressor T cells : Evidence for T cell receptor structures. In: European Journal of Immunology. 1985 ; Vol. 15, No. 8. pp. 855-860.
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