Disuse deterioration of human skeletal muscle challenged by resistive exercise superimposed with vibration: Evidence from structural and proteomic analysis

Michele Salanova, Cecilia Gelfi, Manuela Moriggi, Michele Vasso, Agnese Viganò, Luigi Minafra, Gaetano Bonifacio, Gudrun Schiffl, Martina Gutsmann, Dieter Felsenberg, Paolo Cerretelli, Dieter Blottner

Research output: Contribution to journalArticle

Abstract

In the present bed rest (BR) study, 23 volunteers were randomized into 3 subgroups: 60 d BR control (Ctr); BR with resistive exercise (RE; lowerlimb load); and resistive vibration exercise (RVE; RE with superimposed vibration). The aim was to analyze by confocal and electron microscopy the effects of vibration on myofibril and filament integrity in soleus (Sol) and vastus lateralis (VL) muscle; differential proteomics of contractile, cytoskeletal, and costameric proteins (TN-C, ROCK1, and FAK); and expression of PGC1α and atrophy-related master genes MuRF1 and MuRF2. RVE (but not RE) preserved myofiber size and phenotype in Sol and VL by overexpressing MYBPC1 (42%, P≥0.01), WDR1 (39%, P≥0.01), sarcosin (84%, P≥0.01), and CKM (20%, P≥0.01) and prevented myofibrillar ultrastructural damage as detectable by MuRF1 expression. In Sol, cytoskeletal and contractile proteins were normalized by RVE, and TN-C increased (59%, P≥0.01); the latter also with RE (108%, P≥0.01). In VL, the outcomes of both RVE (acting on sarcosin and desmin) and RE (by way of troponinT-slow and MYL2) were similar. RVE appears to be a highly efficient countermeasure protocol against muscle atrophy and ultrastructural and molecular dysregulation induced by chronic disuse.-Salanova, M., Gelfi, C., Moriggi, M., Vasso, M., Viganò, A., Minafra, L., Bonifacio, G., Schiffl, G., Gutsmann, M., Felsenberg, D., Cerretelli, P., Blottner, D. Disuse deterioration of human skeletal muscle challenged by resistive exercise superimposed with vibration: evidence from structural and proteomic analysis.

Original languageEnglish
Pages (from-to)4748-4763
Number of pages16
JournalFASEB Journal
Volume28
Issue number11
DOIs
Publication statusPublished - Nov 1 2014

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Keywords

  • Atrophy
  • Bed rest
  • Countermeasures
  • Protein expression
  • Ultrastructure

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Molecular Biology
  • Medicine(all)

Cite this

Salanova, M., Gelfi, C., Moriggi, M., Vasso, M., Viganò, A., Minafra, L., Bonifacio, G., Schiffl, G., Gutsmann, M., Felsenberg, D., Cerretelli, P., & Blottner, D. (2014). Disuse deterioration of human skeletal muscle challenged by resistive exercise superimposed with vibration: Evidence from structural and proteomic analysis. FASEB Journal, 28(11), 4748-4763. https://doi.org/10.1096/fj.14-252825