Abstract
Aldolase C is selectively expressed in the hippocampus and Purkinje cells in adult mammalian brain. The gene promoter regions governing cell-specific aldolase C expression are obscure. We show that aldolase C messenger expression in the hippocampus is restricted to CA3 neurons. The human distal promoter region (-200/-1200 bp) is essential for β-galactosidase (β-gal) expression in CA3 neurons and drives high stripe-like β-gal expression in Purkinje cells. The 200 bp proximal promoter region is sufficient to drive low brain-specific and stripe-like β-gal expression in Purkinje cells. Thus, the human aldolase C gene sequences studied drive endogenous-like expression in the brain.
Original language | English |
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Pages (from-to) | 337-344 |
Number of pages | 8 |
Journal | FEBS Letters |
Volume | 578 |
Issue number | 3 |
DOIs | |
Publication status | Published - Dec 17 2004 |
Keywords
- Hippocampus
- Human aldolase C gene
- Purkinje cells
- Transgenic mice
- Zebrin
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology