Diverse human aldolase C gene promoter regions are required to direct specific LacZ expression in the hippocampus and Purkinje cells of transgenic mice

Pasqualina Buono, Ottavia Barbieri, Andreina Alfieri, Annamaria Rosica, Simonetta Astigiano, Daniela Cantatore, Annamaria Mancini, Olimpia Fattoruso, Francesco Salvatore

Research output: Contribution to journalArticlepeer-review

Abstract

Aldolase C is selectively expressed in the hippocampus and Purkinje cells in adult mammalian brain. The gene promoter regions governing cell-specific aldolase C expression are obscure. We show that aldolase C messenger expression in the hippocampus is restricted to CA3 neurons. The human distal promoter region (-200/-1200 bp) is essential for β-galactosidase (β-gal) expression in CA3 neurons and drives high stripe-like β-gal expression in Purkinje cells. The 200 bp proximal promoter region is sufficient to drive low brain-specific and stripe-like β-gal expression in Purkinje cells. Thus, the human aldolase C gene sequences studied drive endogenous-like expression in the brain.

Original languageEnglish
Pages (from-to)337-344
Number of pages8
JournalFEBS Letters
Volume578
Issue number3
DOIs
Publication statusPublished - Dec 17 2004

Keywords

  • Hippocampus
  • Human aldolase C gene
  • Purkinje cells
  • Transgenic mice
  • Zebrin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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