TY - JOUR
T1 - DNA adducts and lung cancer risk
T2 - A prospective study
AU - Peluso, Marco
AU - Munnia, Armelle
AU - Hoek, Gerard
AU - Krzyzanowski, Michal
AU - Veglia, Fabrizio
AU - Airoldi, Luisa
AU - Autrup, Herman
AU - Dunning, Alison
AU - Garte, Seymour
AU - Hainaut, Pierre
AU - Malaveille, Christian
AU - Gormally, Emmanuelle
AU - Matullo, Giuseppe
AU - Overvad, Kim
AU - Raaschou-Nielsen, Ole
AU - Clavel-Chapelon, Francoise
AU - Linseisen, Jacob
AU - Boeing, Heiner
AU - Trichopoulou, Antonia
AU - Trichopoulos, Dimitrios
AU - Kaladidi, Anna
AU - Palli, Domenico
AU - Krogh, Vittorio
AU - Tumino, Rosario
AU - Panico, Salvatore
AU - Bueno-De-Mesquita, H. Bas
AU - Peeters, Petra H.
AU - Kumle, Merethe
AU - Gonzalez, Carlos A.
AU - Martinez, Carmen
AU - Dorronsoro, Miren
AU - Barricarte, Aurelio
AU - Navarro, Carmen
AU - Quiros, J. Ramón
AU - Berglund, Goran
AU - Janzon, Lars
AU - Jarvholm, Bengt
AU - Day, Nicholas E.
AU - Key, Tim J.
AU - Saracci, Rodolfo
AU - Kaaks, Rudolf
AU - Riboli, Elio
AU - Vineis, Paolo
PY - 2005/9/1
Y1 - 2005/9/1
N2 - Objectives were to investigate prospectively the ability of DNA adducts to predict cancer and to study the determinants of adducts, especially air pollutants. DNA adducts were measured in a case-control study nested in the European Prospective Investigation into Cancer and Nutrition (EPIC) investigation. Cases included newly diagnosed lung cancer (n = 115), upper respiratory cancers (pharynx and larynx; n = 82), bladder cancer (n = 124), leukemia (n = 166), and chronic obstructive pulmonary disease or emphysema deaths (n = 77) accrued after a median follow-up of 7 years among the EPIC former smokers and never-smokers. Three controls per case were matched for questionnaire analyses and two controls per case for laboratory analyses. Matching criteria were gender, age, smoking status, country of recruitment, and follow-up time. Individual exposure to air pollution was assessed using concentration data from monitoring stations in routine air quality monitoring networks. Leukocyte DNA adducts were analyzed blindly using 32P postlabeling technique. Adducts were associated with the subsequent risk of lung cancer, with an odds ratio (OR) of 1.86 [95% confidence interval (95% CI), 0.88-3.93] when comparing detectable versus nondetectable adducts. The association with lung cancer was stronger in never-smokers (OR, 4.04; 95% CI, 1.06-15.42) and among the younger age groups. After exclusion of the cancers occurring in the first 36 months of follow-up, the OR was 4.16 (95% CI, 1.24-13.88). A positive association was found between DNA adducts and ozone (O 3) concentration. Our prospective study suggests that leukocyte DNA adducts may predict lung cancer risk of never-smokers. Besides, the association of DNA adduct levels with O 3 indicates a possible role for photochemical smog in determining DNA damage.
AB - Objectives were to investigate prospectively the ability of DNA adducts to predict cancer and to study the determinants of adducts, especially air pollutants. DNA adducts were measured in a case-control study nested in the European Prospective Investigation into Cancer and Nutrition (EPIC) investigation. Cases included newly diagnosed lung cancer (n = 115), upper respiratory cancers (pharynx and larynx; n = 82), bladder cancer (n = 124), leukemia (n = 166), and chronic obstructive pulmonary disease or emphysema deaths (n = 77) accrued after a median follow-up of 7 years among the EPIC former smokers and never-smokers. Three controls per case were matched for questionnaire analyses and two controls per case for laboratory analyses. Matching criteria were gender, age, smoking status, country of recruitment, and follow-up time. Individual exposure to air pollution was assessed using concentration data from monitoring stations in routine air quality monitoring networks. Leukocyte DNA adducts were analyzed blindly using 32P postlabeling technique. Adducts were associated with the subsequent risk of lung cancer, with an odds ratio (OR) of 1.86 [95% confidence interval (95% CI), 0.88-3.93] when comparing detectable versus nondetectable adducts. The association with lung cancer was stronger in never-smokers (OR, 4.04; 95% CI, 1.06-15.42) and among the younger age groups. After exclusion of the cancers occurring in the first 36 months of follow-up, the OR was 4.16 (95% CI, 1.24-13.88). A positive association was found between DNA adducts and ozone (O 3) concentration. Our prospective study suggests that leukocyte DNA adducts may predict lung cancer risk of never-smokers. Besides, the association of DNA adduct levels with O 3 indicates a possible role for photochemical smog in determining DNA damage.
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U2 - 10.1158/0008-5472.CAN-04-3488
DO - 10.1158/0008-5472.CAN-04-3488
M3 - Article
C2 - 16140979
AN - SCOPUS:24744449556
VL - 65
SP - 8042
EP - 8048
JO - Journal of Cancer Research
JF - Journal of Cancer Research
SN - 0008-5472
IS - 17
ER -