Dna complexation by cationic pullulan possessing thermo-sensitive units

M. Constantin, I. Oanea, V. Harabagiu, P. Ascenzi, G. Fundueanu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Cationic pullulan (PN) with pendant thermo-responsive units were complexed with DNA to obtain a potential thermo-sensitive gene carrier. Semitelechelic oligomers of N-isopropylacrylamide/N,N-dim ethylacrylamide possessing -COOH end groups and a lower critical solution temperature of about 36 °C at pH = 7.4 (50 mM phosphate buffer) were prepared by free radical polymerization in the presence of mercaptopropionic acid as the chain transfer agent. Then, the functionalized pullulan with cationic groups was coupled with thermo-responsive oligomers by reaction between the -COOH end groups of oligomers and the remaining-OH groups of PN. Lastly, the thermo-responsive cationic pullulan chains were complexed with DNA at different charge (+/-) ratios. The size of the complexes ranges between 160 and 510 nm depending on the polymer/DNA charge ratio and temperature. The complexes obtained at a charge (+/-) ratio of 5/1 showing a diameter <200 nm, were stable in the presence of bovine serum albumin, and were unaffected by nuclease degradation.

Original languageEnglish
Pages (from-to)849-861
Number of pages13
JournalDigest Journal of Nanomaterials and Biostructures
Volume6
Issue number2
Publication statusPublished - 2011

Fingerprint

Complexation
Oligomers
DNA
oligomers
deoxyribonucleic acid
nuclease
Free radical polymerization
Temperature
Phosphates
Bovine Serum Albumin
Genes
albumins
Polymerization
genes
serums
free radicals
Free Radicals
Degradation
phosphates
Buffers

Keywords

  • DNA complexes
  • Poly(N-isopropylacrylamide) copolymers
  • Potential gene carrier
  • Pullulan
  • Smart polymers

ASJC Scopus subject areas

  • Materials Science(all)
  • Biomedical Engineering
  • Physical and Theoretical Chemistry
  • Condensed Matter Physics
  • Structural Biology
  • Atomic and Molecular Physics, and Optics

Cite this

Constantin, M., Oanea, I., Harabagiu, V., Ascenzi, P., & Fundueanu, G. (2011). Dna complexation by cationic pullulan possessing thermo-sensitive units. Digest Journal of Nanomaterials and Biostructures, 6(2), 849-861.

Dna complexation by cationic pullulan possessing thermo-sensitive units. / Constantin, M.; Oanea, I.; Harabagiu, V.; Ascenzi, P.; Fundueanu, G.

In: Digest Journal of Nanomaterials and Biostructures, Vol. 6, No. 2, 2011, p. 849-861.

Research output: Contribution to journalArticle

Constantin, M, Oanea, I, Harabagiu, V, Ascenzi, P & Fundueanu, G 2011, 'Dna complexation by cationic pullulan possessing thermo-sensitive units', Digest Journal of Nanomaterials and Biostructures, vol. 6, no. 2, pp. 849-861.
Constantin M, Oanea I, Harabagiu V, Ascenzi P, Fundueanu G. Dna complexation by cationic pullulan possessing thermo-sensitive units. Digest Journal of Nanomaterials and Biostructures. 2011;6(2):849-861.
Constantin, M. ; Oanea, I. ; Harabagiu, V. ; Ascenzi, P. ; Fundueanu, G. / Dna complexation by cationic pullulan possessing thermo-sensitive units. In: Digest Journal of Nanomaterials and Biostructures. 2011 ; Vol. 6, No. 2. pp. 849-861.
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