DNA copy number profile discriminates between esophageal adenocarcinoma and squamous cell carcinoma and represents an independent prognostic parameter in esophageal adenocarcinoma

Enrica Rumiato, Giulia Pasello, Marco Montagna, Maria Chiara Scaini, Gian Luca De Salvo, Anna Parenti, Matteo Cagol, Alberto Ruol, Ermanno Ancona, Alberto Amadori, Daniela Saggioro

Research output: Contribution to journalArticle

Abstract

We report multiplex ligation-dependent probe amplification analysis (MLPA) of DNA copy number alterations in 59 esophageal cancer samples, stratified by histotype. Results showed that squamous cell carcinoma (SCC) samples present clustered abnormalities with several genes altered at high frequency. Instead, esophageal adenocarcinoma (ADC) samples are characterized by a more widespread genomic instability, and in these patients total DNA copy number alterations resulted to be an independent prognostic factor. The detection of characteristic molecular changes represents a step towards a better understanding of the molecular basis of esophageal tumorigenesis, and might offer the potential for the discovery of tumor-specific biomarkers.

Original languageEnglish
Pages (from-to)84-93
Number of pages10
JournalCancer Letters
Volume310
Issue number1
DOIs
Publication statusPublished - Nov 1 2011

Fingerprint

Adenocarcinoma
Genomic Instability
Multiplex Polymerase Chain Reaction
DNA
Esophageal Neoplasms
Tumor Biomarkers
Squamous Cell Carcinoma
Carcinogenesis
Genes
Esophageal Squamous Cell Carcinoma

Keywords

  • ADC
  • DNA copy number
  • Esophageal adenocarcinoma
  • Esophageal cancer
  • Esophageal squamous cell carcinoma
  • MLPA
  • SCC

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

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abstract = "We report multiplex ligation-dependent probe amplification analysis (MLPA) of DNA copy number alterations in 59 esophageal cancer samples, stratified by histotype. Results showed that squamous cell carcinoma (SCC) samples present clustered abnormalities with several genes altered at high frequency. Instead, esophageal adenocarcinoma (ADC) samples are characterized by a more widespread genomic instability, and in these patients total DNA copy number alterations resulted to be an independent prognostic factor. The detection of characteristic molecular changes represents a step towards a better understanding of the molecular basis of esophageal tumorigenesis, and might offer the potential for the discovery of tumor-specific biomarkers.",
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AU - Rumiato, Enrica

AU - Pasello, Giulia

AU - Montagna, Marco

AU - Scaini, Maria Chiara

AU - De Salvo, Gian Luca

AU - Parenti, Anna

AU - Cagol, Matteo

AU - Ruol, Alberto

AU - Ancona, Ermanno

AU - Amadori, Alberto

AU - Saggioro, Daniela

PY - 2011/11/1

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N2 - We report multiplex ligation-dependent probe amplification analysis (MLPA) of DNA copy number alterations in 59 esophageal cancer samples, stratified by histotype. Results showed that squamous cell carcinoma (SCC) samples present clustered abnormalities with several genes altered at high frequency. Instead, esophageal adenocarcinoma (ADC) samples are characterized by a more widespread genomic instability, and in these patients total DNA copy number alterations resulted to be an independent prognostic factor. The detection of characteristic molecular changes represents a step towards a better understanding of the molecular basis of esophageal tumorigenesis, and might offer the potential for the discovery of tumor-specific biomarkers.

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