DNA damage-activated kinase Chk2 is independent of proliferation or differentiation yet correlates with tissue biology

C. Lukas, J. Bartkova, L. Latella, J. Falck, N. Mailand, T. Schroeder, M. Sehested, J. Lukas, J. Bartek

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

The Chk2 kinase is a tumor suppressor and key transducer of DNA-damage checkpoints. We show that the human Chk2 protein is relatively stable, nuclear, and responding to γ-radiation throughout the cell cycle. Contrary to the retinoblastoma protein-regulated, labile Chk1 kinase restricted to S-G 2 phases, Chk2 remains activatable even in quiescent and differentiating cells. In human tissues, Chk2 is homogeneously expressed in renewing cell populations such as epidermis or intestine, heterogeneous in conditionally renewing tissues, and absent or cytoplasmic in static tissues such as muscle or brain. These data highlight striking differences between Chk2 and Chk1 and show unexpected correlation of Chk2 expression with tissue biology.

Original languageEnglish
Pages (from-to)4990-4993
Number of pages4
JournalCancer Research
Volume61
Issue number13
Publication statusPublished - Jul 1 2001

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DNA Damage
Phosphotransferases
Retinoblastoma Protein
Transducers
Epidermis
Intestines
Cell Cycle
Radiation
Muscles
Brain
Population
Neoplasms
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Lukas, C., Bartkova, J., Latella, L., Falck, J., Mailand, N., Schroeder, T., ... Bartek, J. (2001). DNA damage-activated kinase Chk2 is independent of proliferation or differentiation yet correlates with tissue biology. Cancer Research, 61(13), 4990-4993.

DNA damage-activated kinase Chk2 is independent of proliferation or differentiation yet correlates with tissue biology. / Lukas, C.; Bartkova, J.; Latella, L.; Falck, J.; Mailand, N.; Schroeder, T.; Sehested, M.; Lukas, J.; Bartek, J.

In: Cancer Research, Vol. 61, No. 13, 01.07.2001, p. 4990-4993.

Research output: Contribution to journalArticle

Lukas, C, Bartkova, J, Latella, L, Falck, J, Mailand, N, Schroeder, T, Sehested, M, Lukas, J & Bartek, J 2001, 'DNA damage-activated kinase Chk2 is independent of proliferation or differentiation yet correlates with tissue biology', Cancer Research, vol. 61, no. 13, pp. 4990-4993.
Lukas, C. ; Bartkova, J. ; Latella, L. ; Falck, J. ; Mailand, N. ; Schroeder, T. ; Sehested, M. ; Lukas, J. ; Bartek, J. / DNA damage-activated kinase Chk2 is independent of proliferation or differentiation yet correlates with tissue biology. In: Cancer Research. 2001 ; Vol. 61, No. 13. pp. 4990-4993.
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