DNA damage and genomic instability among workers formerly and currently exposed to asbestos

Mirta Milić, Monica Neri, Marcello Ceppi, Marco Bruzzone, Armelle Munnia, Donatella Ugolini, Alfonso Cristaudo, Alessandra Bonotti, Marco E. Peluso, Stefano Bonassi

Research output: Contribution to journalArticlepeer-review


Objectives Despite an asbestos ban in the European Union, exposure to asbestos still represents an occupational risk. Biomarkers of DNA damage and genomic instability in groups exposed to asbestos may contribute to the identification of subgroups/subjects at higher risk. Methods A cross-sectional study was conducted on 468 male individuals (80 working in occupational settings with potential exposure to asbestos fibers, 202 retired workers with past exposure, and 186 non-exposed controls) to compare genomic instability, cell proliferation and differentiation level using the non-invasive micronucleus buccal cytome assay. Data on demographic variables, lifestyle, and occupational history were collected with a standardized questionnaire. Micronuclei (MN) and other biomarkers of DNA damage and genomic instability were scored in a minimum of 2000/1000 cells per individual, respectively. Results Univariate and multivariate analysis showed opposite associations of MN frequency with current and former exposure. Compared to unexposed controls, workers with current potential exposure to asbestos had 55% lower MN frequency [95% confidence interval (CI) 71–29%, P<0.001] while those with past exposure had 34% higher MN frequency (95% CI 1–77%, P<0.001). The frequency of cells with condensed chromatin and binucleated cells was elevated among formerly exposed workers. The multivariate analysis did not reveal any actual confounders, although lower MN frequency was observed among subjects eating fresh fruit or vegetables every day or taking vitamin supplements. Conclusions Active workers with potential exposure to asbestos fibers did not show increased genomic damage. On the contrary, workers exposed in the past experienced a persistently elevated genomic instability, which may be used for risk assessment at subgroup or individual level.

Original languageEnglish
Pages (from-to)423-431
Number of pages9
JournalScandinavian Journal of Work, Environment and Health
Issue number4
Publication statusPublished - Jan 1 2018


  • Asbestos
  • Biomarker
  • Buccal mucosa
  • DNA
  • DNA damage
  • Genomic instability
  • Micronucleus cytome assay
  • Mutagenicity test
  • Occupational exposure

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health


Dive into the research topics of 'DNA damage and genomic instability among workers formerly and currently exposed to asbestos'. Together they form a unique fingerprint.

Cite this