DNA damage by tobacco smoke and some antiblastic drugs evaluated using the Comet assay

P. Poli, A. Buschini, A. Spaggiari, V. Rizzoli, C. Carlo-Stella, C. Rossi

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The Comet assay was used in human leukocytes to detect, in vivo, DNA strand-breaks induced by smoking habit to evaluate the test sensitivity to an environmental factor, and by several antiblastic drugs to note their effectiveness at single cell level. Differences related to smoking habit, gender and age are evident. Melphalan shows the widest DNA damage. The damage induced by etoposide can be ascribed to the balancing between the production of strand-breaks and cross-links which limit the migration of DNA fragments. Interferon, fludarabine, prednisone, and oncocarbide appear to induce unexpected strand-breaks. Single cell gel electrophoresis (SCGE) is highly effective in revealing the association between DNA damage and environmental, genetic, and acquired factors, providing further data on the possible applicability of this assay in genotoxic human surveillance in addition to established tests. Moreover, the ability to point out cell subpopulations varying in mean damage levels could allow detection of potentially emerging drug-resistant populations.

Original languageEnglish
Pages (from-to)267-276
Number of pages10
JournalToxicology Letters
Volume108
Issue number2-3
DOIs
Publication statusPublished - Sep 5 1999

Fingerprint

Comet Assay
Tobacco
Smoke
DNA Damage
Habits
Assays
Smoking
DNA Breaks
Aptitude
Melphalan
Hydroxyurea
DNA
Etoposide
Prednisone
Pharmaceutical Preparations
Interferons
Leukocytes
Electrophoresis
Population
Gels

Keywords

  • Antiblastic drugs
  • Biomarker
  • DNA-strand break
  • Environmental exposure

ASJC Scopus subject areas

  • Toxicology

Cite this

DNA damage by tobacco smoke and some antiblastic drugs evaluated using the Comet assay. / Poli, P.; Buschini, A.; Spaggiari, A.; Rizzoli, V.; Carlo-Stella, C.; Rossi, C.

In: Toxicology Letters, Vol. 108, No. 2-3, 05.09.1999, p. 267-276.

Research output: Contribution to journalArticle

Poli, P. ; Buschini, A. ; Spaggiari, A. ; Rizzoli, V. ; Carlo-Stella, C. ; Rossi, C. / DNA damage by tobacco smoke and some antiblastic drugs evaluated using the Comet assay. In: Toxicology Letters. 1999 ; Vol. 108, No. 2-3. pp. 267-276.
@article{88e6f9e39e3241858c920ac08a1def96,
title = "DNA damage by tobacco smoke and some antiblastic drugs evaluated using the Comet assay",
abstract = "The Comet assay was used in human leukocytes to detect, in vivo, DNA strand-breaks induced by smoking habit to evaluate the test sensitivity to an environmental factor, and by several antiblastic drugs to note their effectiveness at single cell level. Differences related to smoking habit, gender and age are evident. Melphalan shows the widest DNA damage. The damage induced by etoposide can be ascribed to the balancing between the production of strand-breaks and cross-links which limit the migration of DNA fragments. Interferon, fludarabine, prednisone, and oncocarbide appear to induce unexpected strand-breaks. Single cell gel electrophoresis (SCGE) is highly effective in revealing the association between DNA damage and environmental, genetic, and acquired factors, providing further data on the possible applicability of this assay in genotoxic human surveillance in addition to established tests. Moreover, the ability to point out cell subpopulations varying in mean damage levels could allow detection of potentially emerging drug-resistant populations.",
keywords = "Antiblastic drugs, Biomarker, DNA-strand break, Environmental exposure",
author = "P. Poli and A. Buschini and A. Spaggiari and V. Rizzoli and C. Carlo-Stella and C. Rossi",
year = "1999",
month = "9",
day = "5",
doi = "10.1016/S0378-4274(99)00098-3",
language = "English",
volume = "108",
pages = "267--276",
journal = "Toxicology Letters",
issn = "0378-4274",
publisher = "Elsevier BV",
number = "2-3",

}

TY - JOUR

T1 - DNA damage by tobacco smoke and some antiblastic drugs evaluated using the Comet assay

AU - Poli, P.

AU - Buschini, A.

AU - Spaggiari, A.

AU - Rizzoli, V.

AU - Carlo-Stella, C.

AU - Rossi, C.

PY - 1999/9/5

Y1 - 1999/9/5

N2 - The Comet assay was used in human leukocytes to detect, in vivo, DNA strand-breaks induced by smoking habit to evaluate the test sensitivity to an environmental factor, and by several antiblastic drugs to note their effectiveness at single cell level. Differences related to smoking habit, gender and age are evident. Melphalan shows the widest DNA damage. The damage induced by etoposide can be ascribed to the balancing between the production of strand-breaks and cross-links which limit the migration of DNA fragments. Interferon, fludarabine, prednisone, and oncocarbide appear to induce unexpected strand-breaks. Single cell gel electrophoresis (SCGE) is highly effective in revealing the association between DNA damage and environmental, genetic, and acquired factors, providing further data on the possible applicability of this assay in genotoxic human surveillance in addition to established tests. Moreover, the ability to point out cell subpopulations varying in mean damage levels could allow detection of potentially emerging drug-resistant populations.

AB - The Comet assay was used in human leukocytes to detect, in vivo, DNA strand-breaks induced by smoking habit to evaluate the test sensitivity to an environmental factor, and by several antiblastic drugs to note their effectiveness at single cell level. Differences related to smoking habit, gender and age are evident. Melphalan shows the widest DNA damage. The damage induced by etoposide can be ascribed to the balancing between the production of strand-breaks and cross-links which limit the migration of DNA fragments. Interferon, fludarabine, prednisone, and oncocarbide appear to induce unexpected strand-breaks. Single cell gel electrophoresis (SCGE) is highly effective in revealing the association between DNA damage and environmental, genetic, and acquired factors, providing further data on the possible applicability of this assay in genotoxic human surveillance in addition to established tests. Moreover, the ability to point out cell subpopulations varying in mean damage levels could allow detection of potentially emerging drug-resistant populations.

KW - Antiblastic drugs

KW - Biomarker

KW - DNA-strand break

KW - Environmental exposure

UR - http://www.scopus.com/inward/record.url?scp=0032860529&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032860529&partnerID=8YFLogxK

U2 - 10.1016/S0378-4274(99)00098-3

DO - 10.1016/S0378-4274(99)00098-3

M3 - Article

C2 - 10511271

AN - SCOPUS:0032860529

VL - 108

SP - 267

EP - 276

JO - Toxicology Letters

JF - Toxicology Letters

SN - 0378-4274

IS - 2-3

ER -