DNA damage in mouse and rat liver by caprolactam and benzoin, evaluated with three different methods

Silvio Parodi, Maria Luisa Abelmoschi, Cecilia Balbi, Maria Teresa De Angeli, Mauro Pala, Patrizia Russo, Maurizio Taningher, Leonardo Santi

Research output: Contribution to journalArticlepeer-review

Abstract

Benzoin and caprolactam were examined for their capability of inducing alkaline DNA fragmentation in mouse and rat liver DNA after treatment in vivo. Three different methods were used. With the alkaline elution technique we measured an effect presumably related to the conformation of the DNA coil. With a viscometric and a fluorometric unwinding method we measured an effect presumably related to the number of unwinding points in DNA. For both compounds only the alkaline elution technique was clearly positive. The results suggest that both caprolactam and benzoin can induce an important change in the conformation of the DNA coil without inducing true breaks in DNA.

Original languageEnglish
Pages (from-to)379-384
Number of pages6
JournalMutation Research/Genetic Toxicology
Volume224
Issue number3
DOIs
Publication statusPublished - 1989

Keywords

  • Benzoin
  • Caprolactam
  • DNA damage

ASJC Scopus subject areas

  • Genetics
  • Toxicology
  • Medicine(all)

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