DNA demethylation is directly related to tumour progression: Evidence in normal, pre-malignant and malignant cells from uterine cervix samples

Adriana De Capoa, Angelina Musolino, Simonetta Della Rosa, Paola Caiafa, Luciano Mariani, Franca Del Nonno, Amina Vocaturo, Raffaele Perrone Donnorso, Alain Niveleau, Claudio Grappelli

Research output: Contribution to journalArticle

Abstract

A computer-assisted assay based on the quantitative analysis of DNA methylation in individual interphase nuclei by indirect immunolabelling with anti-5-methylcytosine antibodies was recently developed in our laboratory. In situ analyses were performed on individual nuclei from normal and experimentally hypo- or hypermethylated cultured cells as well as on human peripheral blood B-lymphocytes from normal and chronic lymphoid leukemia (CLL) samples. We present the results obtained on cells from patients affected by different degrees of preneoplastic or neoplastic changes of the uterine cervix as compared to normal controls. The analysis of DNA methylation in individual cells from cytofuge samples was performed as follows: within each nucleus the eu- and heterochromatin methylation levels were quantified in the grey scale range by dedicated software in terms of numbers, areas and optical densities (ODs) of the immunolabeled dense heterochromatic regions ('spots'), and of the optical density of nuclear background, i.e., of nuclear euchromatin. Analogously, in randomly chosen microscope fields of tissue sections from paraffin-embedded samples, progressive tissue demethylation was observed in dysplastic and cancer cells as compared to normal ones. Both methods showed significant and progressive DNA hypomethylation in dysplastic and cancer cells as compared to control specimens.

Original languageEnglish
Pages (from-to)545-549
Number of pages5
JournalOncology Reports
Volume10
Issue number3
Publication statusPublished - May 2003

Fingerprint

Cervix Uteri
Euchromatin
DNA
DNA Methylation
Neoplasms
5-Methylcytosine
Lymphoid Leukemia
Heterochromatin
Interphase
Paraffin
Methylation
Cultured Cells
B-Lymphocytes
Software
Antibodies

Keywords

  • DNA demethylation
  • Uterine cervix

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

DNA demethylation is directly related to tumour progression : Evidence in normal, pre-malignant and malignant cells from uterine cervix samples. / De Capoa, Adriana; Musolino, Angelina; Della Rosa, Simonetta; Caiafa, Paola; Mariani, Luciano; Del Nonno, Franca; Vocaturo, Amina; Donnorso, Raffaele Perrone; Niveleau, Alain; Grappelli, Claudio.

In: Oncology Reports, Vol. 10, No. 3, 05.2003, p. 545-549.

Research output: Contribution to journalArticle

De Capoa, A, Musolino, A, Della Rosa, S, Caiafa, P, Mariani, L, Del Nonno, F, Vocaturo, A, Donnorso, RP, Niveleau, A & Grappelli, C 2003, 'DNA demethylation is directly related to tumour progression: Evidence in normal, pre-malignant and malignant cells from uterine cervix samples', Oncology Reports, vol. 10, no. 3, pp. 545-549.
De Capoa, Adriana ; Musolino, Angelina ; Della Rosa, Simonetta ; Caiafa, Paola ; Mariani, Luciano ; Del Nonno, Franca ; Vocaturo, Amina ; Donnorso, Raffaele Perrone ; Niveleau, Alain ; Grappelli, Claudio. / DNA demethylation is directly related to tumour progression : Evidence in normal, pre-malignant and malignant cells from uterine cervix samples. In: Oncology Reports. 2003 ; Vol. 10, No. 3. pp. 545-549.
@article{65bf23bdbb784c9fa21272f2f5f62963,
title = "DNA demethylation is directly related to tumour progression: Evidence in normal, pre-malignant and malignant cells from uterine cervix samples",
abstract = "A computer-assisted assay based on the quantitative analysis of DNA methylation in individual interphase nuclei by indirect immunolabelling with anti-5-methylcytosine antibodies was recently developed in our laboratory. In situ analyses were performed on individual nuclei from normal and experimentally hypo- or hypermethylated cultured cells as well as on human peripheral blood B-lymphocytes from normal and chronic lymphoid leukemia (CLL) samples. We present the results obtained on cells from patients affected by different degrees of preneoplastic or neoplastic changes of the uterine cervix as compared to normal controls. The analysis of DNA methylation in individual cells from cytofuge samples was performed as follows: within each nucleus the eu- and heterochromatin methylation levels were quantified in the grey scale range by dedicated software in terms of numbers, areas and optical densities (ODs) of the immunolabeled dense heterochromatic regions ('spots'), and of the optical density of nuclear background, i.e., of nuclear euchromatin. Analogously, in randomly chosen microscope fields of tissue sections from paraffin-embedded samples, progressive tissue demethylation was observed in dysplastic and cancer cells as compared to normal ones. Both methods showed significant and progressive DNA hypomethylation in dysplastic and cancer cells as compared to control specimens.",
keywords = "DNA demethylation, Uterine cervix",
author = "{De Capoa}, Adriana and Angelina Musolino and {Della Rosa}, Simonetta and Paola Caiafa and Luciano Mariani and {Del Nonno}, Franca and Amina Vocaturo and Donnorso, {Raffaele Perrone} and Alain Niveleau and Claudio Grappelli",
year = "2003",
month = "5",
language = "English",
volume = "10",
pages = "545--549",
journal = "Oncology Reports",
issn = "1021-335X",
publisher = "Spandidos Publications",
number = "3",

}

TY - JOUR

T1 - DNA demethylation is directly related to tumour progression

T2 - Evidence in normal, pre-malignant and malignant cells from uterine cervix samples

AU - De Capoa, Adriana

AU - Musolino, Angelina

AU - Della Rosa, Simonetta

AU - Caiafa, Paola

AU - Mariani, Luciano

AU - Del Nonno, Franca

AU - Vocaturo, Amina

AU - Donnorso, Raffaele Perrone

AU - Niveleau, Alain

AU - Grappelli, Claudio

PY - 2003/5

Y1 - 2003/5

N2 - A computer-assisted assay based on the quantitative analysis of DNA methylation in individual interphase nuclei by indirect immunolabelling with anti-5-methylcytosine antibodies was recently developed in our laboratory. In situ analyses were performed on individual nuclei from normal and experimentally hypo- or hypermethylated cultured cells as well as on human peripheral blood B-lymphocytes from normal and chronic lymphoid leukemia (CLL) samples. We present the results obtained on cells from patients affected by different degrees of preneoplastic or neoplastic changes of the uterine cervix as compared to normal controls. The analysis of DNA methylation in individual cells from cytofuge samples was performed as follows: within each nucleus the eu- and heterochromatin methylation levels were quantified in the grey scale range by dedicated software in terms of numbers, areas and optical densities (ODs) of the immunolabeled dense heterochromatic regions ('spots'), and of the optical density of nuclear background, i.e., of nuclear euchromatin. Analogously, in randomly chosen microscope fields of tissue sections from paraffin-embedded samples, progressive tissue demethylation was observed in dysplastic and cancer cells as compared to normal ones. Both methods showed significant and progressive DNA hypomethylation in dysplastic and cancer cells as compared to control specimens.

AB - A computer-assisted assay based on the quantitative analysis of DNA methylation in individual interphase nuclei by indirect immunolabelling with anti-5-methylcytosine antibodies was recently developed in our laboratory. In situ analyses were performed on individual nuclei from normal and experimentally hypo- or hypermethylated cultured cells as well as on human peripheral blood B-lymphocytes from normal and chronic lymphoid leukemia (CLL) samples. We present the results obtained on cells from patients affected by different degrees of preneoplastic or neoplastic changes of the uterine cervix as compared to normal controls. The analysis of DNA methylation in individual cells from cytofuge samples was performed as follows: within each nucleus the eu- and heterochromatin methylation levels were quantified in the grey scale range by dedicated software in terms of numbers, areas and optical densities (ODs) of the immunolabeled dense heterochromatic regions ('spots'), and of the optical density of nuclear background, i.e., of nuclear euchromatin. Analogously, in randomly chosen microscope fields of tissue sections from paraffin-embedded samples, progressive tissue demethylation was observed in dysplastic and cancer cells as compared to normal ones. Both methods showed significant and progressive DNA hypomethylation in dysplastic and cancer cells as compared to control specimens.

KW - DNA demethylation

KW - Uterine cervix

UR - http://www.scopus.com/inward/record.url?scp=0642345170&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0642345170&partnerID=8YFLogxK

M3 - Article

C2 - 12684621

AN - SCOPUS:0642345170

VL - 10

SP - 545

EP - 549

JO - Oncology Reports

JF - Oncology Reports

SN - 1021-335X

IS - 3

ER -