DNA DSB induced by iron ions in human fibroblasts: LET dependence and shielding efficiency

G. Esposito, F. Antonelli, M. Belli, A. Campa, V. Dini, Y. Furusawa, G. Simone, E. Sorrentino, M. A. Tabocchini

Research output: Contribution to journalArticle

Abstract

This paper reports on DNA DSB induction in human fibroblasts by iron ions of different energies, namely 5, 1 GeV/u, 414 and 115 MeV/u, in absence or presence of different shields (PMMA, Al and Pb). Measure of DNA DSB was performed by calibrated Pulsed Field Gel Electrophoresis using the fragment counting method. The RBE-LET relationships for unshielded and shielded beams were obtained both in terms of dose average LET and of track average LET. Weak dependence on these parameters was observed for DSB induction. The shielding efficiency, evaluated by the ratio between the cross sections for unshielded and shielded beams, depends not only on the shield type and thickness, but also on the beam energy. Protection is only observed at high iron ions energy, especially at 5 GeV/u, where PMMA shield gives higher protection compared to Al or Pb shields of the same thickness expressed in g/cm 2 .

Original languageEnglish
Pages (from-to)243-248
Number of pages6
JournalAdvances in Space Research
Volume35
Issue number2
DOIs
Publication statusPublished - Jan 1 2005

Keywords

  • DNA DSB
  • HZE particles
  • PFGE
  • Radiation shielding
  • RBE-LET relationship
  • Space radiation

ASJC Scopus subject areas

  • Aerospace Engineering
  • Astronomy and Astrophysics
  • Geophysics
  • Atmospheric Science
  • Space and Planetary Science
  • Earth and Planetary Sciences(all)

Fingerprint Dive into the research topics of 'DNA DSB induced by iron ions in human fibroblasts: LET dependence and shielding efficiency'. Together they form a unique fingerprint.

  • Cite this