DNA DSB induction and rejoining in V79 cells irradiated with light ions: A constant field gel electrophoresis study

M. Belli, R. Cherubini, M. Dalla Vecchia, V. Dini, G. Moschini, C. Signoretti, G. Simone, M. A. Tabocchini, P. Tiveron

Research output: Contribution to journalArticle

Abstract

Purpose: To study the induction and the time-course of rejoining of DNA double strand breaks (DSB) in V79 cells irradiated with light ions with different linear energy transfer (LET). Material and methods: V79 cells were irradiated in monolayer with monoenergetic proton, deuteron, helium-3 or helium-4 ion beams, each at two different energy values. Gamma rays were used as reference radiation. DSB have been measured by constant field gel electrophoresis (CFGE). Results: The initial yield depended little on the particle type and LET. The amount of DSB left unrejoined for up to 2 h incubation time could be roughly described by a decreasing exponential function with a final plateau, although more complex functions cannot be excluded. Radiation quality had little effect on the rejoining rate but affected the plateau. The amount of residual DSB after 2 h was higher for densely than for sparsely ionizing radiation, and for the same particle was dependent on LET. The corresponding RBE ranged from 1.8 to 6.0. Conclusion: The results support the hypothesis that complex, less reparable DSB are induced in higher proportion by light ions with respect to γ-rays and that, for the same ion, increasing LET leads to an increase in this proportion.

Original languageEnglish
Pages (from-to)1095-1104
Number of pages10
JournalInternational Journal of Radiation Biology
Volume76
Issue number8
DOIs
Publication statusPublished - Jan 1 2000
Externally publishedYes

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

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