DNA elements target of transcriptional factors are not restricted to long terminal repeat of human immunodeficiency virus

G. Feriotto, S. Volinia, P. Giacomini, R. Gambari

Research output: Contribution to journalArticle

Abstract

In this report we show that signals for transcriptional factors are not restricted to the HIV-1 LTR, but are present throughout the HIV-1 genome. Furthermore we identified a sequence AGAACAGATG, highly homologous to the X-box of class II MHC genes and located within the tat-IVS/env region of HIV-1. Double stranded oligonucleotides mimicking the HIV-1 region containing AGAACAGATG were synthesized and band shift experiments were performed demonstrating that this HIV-1 genomic region binds nuclear proteins. We further demonstrate that the binding of nuclear factors to this tat-IVS/env HIV-1 sequence is competed for in the band-shift assay by the highly homologous X-box of the promoter of the human HLA-DRα gene. The presence in the HIV-1 genome of DNA sequences homologous or identical to regulatory sequences of cellular genes represents a potential mechanism of predation of DNA elements recognized by DNA binding proteins.

Original languageEnglish
Pages (from-to)65-71
Number of pages7
JournalAnticancer Research
Volume12
Issue number1
Publication statusPublished - 1992

Keywords

  • AIDS
  • DNA-binding factors
  • HIV-1
  • HLA-DRα
  • Oligonucleotides

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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