DNA end binding activity and Ku70/80 heterodimer expression in human colorectal tumor

Paola Mazzarelli, Paola Parrella, Davide Seripa, Emanuela Signori, Giuseppe Perrone, Carla Rabitti, Domenico Borzomati, Armando Gabbrielli, Maria Giovanna Matera, Carolina Gravina, Marco Caricato, Maria Luana Poeta, Monica Rinaldi, Sergio Valeri, Roberto Coppola, Vito Michele Fazio

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Aim: To determine the DNA binding activity and protein levels of the Ku70/80 heterodimer, the functional mediator of the NHEJ activity, in human colorectal carcinogenesis. Methods: The Ku70/80 DNA-binding activity was determined by electrophoretic mobility shift assays in 20 colon adenoma and 15 colorectal cancer samples as well as matched normal colonic tissues. Nuclear and cytoplasmic protein expression was determined by immunohistochemistry and Western blot analysis. Results: A statistically significant difference was found in both adenomas and carcinomas as compared to matched normal colonic mucosa (P

Original languageEnglish
Pages (from-to)6694-6700
Number of pages7
JournalWorld Journal of Gastroenterology
Volume11
Issue number42
Publication statusPublished - Nov 14 2005

Fingerprint

Adenoma
Colorectal Neoplasms
DNA
DNA-Binding Proteins
Electrophoretic Mobility Shift Assay
Nuclear Proteins
Human Activities
Colon
Carcinogenesis
Mucous Membrane
Western Blotting
Immunohistochemistry
Carcinoma

Keywords

  • Chromosomal instability
  • Colon adenoma
  • Colorectal cancer
  • DNA-dependent protein kinase
  • Double strand break repair
  • Ku70/80 heterodimer
  • Mismatch repair
  • Non-homologous end joining

ASJC Scopus subject areas

  • Gastroenterology

Cite this

DNA end binding activity and Ku70/80 heterodimer expression in human colorectal tumor. / Mazzarelli, Paola; Parrella, Paola; Seripa, Davide; Signori, Emanuela; Perrone, Giuseppe; Rabitti, Carla; Borzomati, Domenico; Gabbrielli, Armando; Matera, Maria Giovanna; Gravina, Carolina; Caricato, Marco; Poeta, Maria Luana; Rinaldi, Monica; Valeri, Sergio; Coppola, Roberto; Fazio, Vito Michele.

In: World Journal of Gastroenterology, Vol. 11, No. 42, 14.11.2005, p. 6694-6700.

Research output: Contribution to journalArticle

Mazzarelli, P, Parrella, P, Seripa, D, Signori, E, Perrone, G, Rabitti, C, Borzomati, D, Gabbrielli, A, Matera, MG, Gravina, C, Caricato, M, Poeta, ML, Rinaldi, M, Valeri, S, Coppola, R & Fazio, VM 2005, 'DNA end binding activity and Ku70/80 heterodimer expression in human colorectal tumor', World Journal of Gastroenterology, vol. 11, no. 42, pp. 6694-6700.
Mazzarelli, Paola ; Parrella, Paola ; Seripa, Davide ; Signori, Emanuela ; Perrone, Giuseppe ; Rabitti, Carla ; Borzomati, Domenico ; Gabbrielli, Armando ; Matera, Maria Giovanna ; Gravina, Carolina ; Caricato, Marco ; Poeta, Maria Luana ; Rinaldi, Monica ; Valeri, Sergio ; Coppola, Roberto ; Fazio, Vito Michele. / DNA end binding activity and Ku70/80 heterodimer expression in human colorectal tumor. In: World Journal of Gastroenterology. 2005 ; Vol. 11, No. 42. pp. 6694-6700.
@article{0bae32bdd7e4438aaa685acfa614892c,
title = "DNA end binding activity and Ku70/80 heterodimer expression in human colorectal tumor",
abstract = "Aim: To determine the DNA binding activity and protein levels of the Ku70/80 heterodimer, the functional mediator of the NHEJ activity, in human colorectal carcinogenesis. Methods: The Ku70/80 DNA-binding activity was determined by electrophoretic mobility shift assays in 20 colon adenoma and 15 colorectal cancer samples as well as matched normal colonic tissues. Nuclear and cytoplasmic protein expression was determined by immunohistochemistry and Western blot analysis. Results: A statistically significant difference was found in both adenomas and carcinomas as compared to matched normal colonic mucosa (P",
keywords = "Chromosomal instability, Colon adenoma, Colorectal cancer, DNA-dependent protein kinase, Double strand break repair, Ku70/80 heterodimer, Mismatch repair, Non-homologous end joining",
author = "Paola Mazzarelli and Paola Parrella and Davide Seripa and Emanuela Signori and Giuseppe Perrone and Carla Rabitti and Domenico Borzomati and Armando Gabbrielli and Matera, {Maria Giovanna} and Carolina Gravina and Marco Caricato and Poeta, {Maria Luana} and Monica Rinaldi and Sergio Valeri and Roberto Coppola and Fazio, {Vito Michele}",
year = "2005",
month = "11",
day = "14",
language = "English",
volume = "11",
pages = "6694--6700",
journal = "World Journal of Gastroenterology",
issn = "1007-9327",
publisher = "WJG Press",
number = "42",

}

TY - JOUR

T1 - DNA end binding activity and Ku70/80 heterodimer expression in human colorectal tumor

AU - Mazzarelli, Paola

AU - Parrella, Paola

AU - Seripa, Davide

AU - Signori, Emanuela

AU - Perrone, Giuseppe

AU - Rabitti, Carla

AU - Borzomati, Domenico

AU - Gabbrielli, Armando

AU - Matera, Maria Giovanna

AU - Gravina, Carolina

AU - Caricato, Marco

AU - Poeta, Maria Luana

AU - Rinaldi, Monica

AU - Valeri, Sergio

AU - Coppola, Roberto

AU - Fazio, Vito Michele

PY - 2005/11/14

Y1 - 2005/11/14

N2 - Aim: To determine the DNA binding activity and protein levels of the Ku70/80 heterodimer, the functional mediator of the NHEJ activity, in human colorectal carcinogenesis. Methods: The Ku70/80 DNA-binding activity was determined by electrophoretic mobility shift assays in 20 colon adenoma and 15 colorectal cancer samples as well as matched normal colonic tissues. Nuclear and cytoplasmic protein expression was determined by immunohistochemistry and Western blot analysis. Results: A statistically significant difference was found in both adenomas and carcinomas as compared to matched normal colonic mucosa (P

AB - Aim: To determine the DNA binding activity and protein levels of the Ku70/80 heterodimer, the functional mediator of the NHEJ activity, in human colorectal carcinogenesis. Methods: The Ku70/80 DNA-binding activity was determined by electrophoretic mobility shift assays in 20 colon adenoma and 15 colorectal cancer samples as well as matched normal colonic tissues. Nuclear and cytoplasmic protein expression was determined by immunohistochemistry and Western blot analysis. Results: A statistically significant difference was found in both adenomas and carcinomas as compared to matched normal colonic mucosa (P

KW - Chromosomal instability

KW - Colon adenoma

KW - Colorectal cancer

KW - DNA-dependent protein kinase

KW - Double strand break repair

KW - Ku70/80 heterodimer

KW - Mismatch repair

KW - Non-homologous end joining

UR - http://www.scopus.com/inward/record.url?scp=30444445713&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=30444445713&partnerID=8YFLogxK

M3 - Article

C2 - 16425368

AN - SCOPUS:30444445713

VL - 11

SP - 6694

EP - 6700

JO - World Journal of Gastroenterology

JF - World Journal of Gastroenterology

SN - 1007-9327

IS - 42

ER -