DNA end binding activity and Ku70/80 heterodimer expression in human colorectal tumor

Paola Mazzarelli, Paola Parrella, Davide Seripa, Emanuela Signori, Giuseppe Perrone, Carla Rabitti, Domenico Borzomati, Armando Gabbrielli, Maria Giovanna Matera, Carolina Gravina, Marco Caricato, Maria Luana Poeta, Monica Rinaldi, Sergio Valeri, Roberto Coppola, Vito Michele Fazio

Research output: Contribution to journalArticle

Abstract

Aim: To determine the DNA binding activity and protein levels of the Ku70/80 heterodimer, the functional mediator of the NHEJ activity, in human colorectal carcinogenesis. Methods: The Ku70/80 DNA-binding activity was determined by electrophoretic mobility shift assays in 20 colon adenoma and 15 colorectal cancer samples as well as matched normal colonic tissues. Nuclear and cytoplasmic protein expression was determined by immunohistochemistry and Western blot analysis. Results: A statistically significant difference was found in both adenomas and carcinomas as compared to matched normal colonic mucosa (P

Original languageEnglish
Pages (from-to)6694-6700
Number of pages7
JournalWorld Journal of Gastroenterology
Volume11
Issue number42
Publication statusPublished - Nov 14 2005

Keywords

  • Chromosomal instability
  • Colon adenoma
  • Colorectal cancer
  • DNA-dependent protein kinase
  • Double strand break repair
  • Ku70/80 heterodimer
  • Mismatch repair
  • Non-homologous end joining

ASJC Scopus subject areas

  • Gastroenterology

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    Mazzarelli, P., Parrella, P., Seripa, D., Signori, E., Perrone, G., Rabitti, C., Borzomati, D., Gabbrielli, A., Matera, M. G., Gravina, C., Caricato, M., Poeta, M. L., Rinaldi, M., Valeri, S., Coppola, R., & Fazio, V. M. (2005). DNA end binding activity and Ku70/80 heterodimer expression in human colorectal tumor. World Journal of Gastroenterology, 11(42), 6694-6700.