DNA fragmentation in V79 cells irradiated with light ions as measured by pulsed-field gel electrophoresis. I. Experimental results

M. Belli, R. Cherubini, M. Dalla Vecchia, Valentina Dini, G. Esposito, G. Moschini, O. Sapora, G. Simone, M. A. Tabocchini

Research output: Contribution to journalArticle

Abstract

Purpose: To compare the results on DNA fragmentation induced in Chinese hamster V79 cells by various doses of γ-rays and low-energy protons and helium-4 ions. Materials and methods: V79 cells were irradiated as monolayers with monoenergetic protons and helium-4 ions; γ-rays were used as the reference radiation. DNA double-strand breaks were evaluated by calibrated pulsed-field gel electrophoresis using conditions covering the range 5.7 Mbp-23.1kbp. Results: The fragment-counting method gave double-strand breaks yields and the relative biological effectiveness higher than those obtained by the fraction of activity released method. The frequency distribution of fragments showed that protons and helium ions induced more fragments below the Mbp region than did γ-rays at the same dose. The distributions for both the irradiated and non-irradiated samples clearly appeared to be non-random. Conclusion: Differences were observed in the yield and spatial correlation, at a molecular size scale characteristic of loop dimensions, of the double-strand breaks induced by γ-rays and by light ions. These effects may have a role in the observed different cell response to these radiations.

Original languageEnglish
Pages (from-to)475-482
Number of pages8
JournalInternational Journal of Radiation Biology
Volume78
Issue number6
DOIs
Publication statusPublished - Jul 4 2002

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

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