DNA fragmentation induced in K562 cells by nitrogen ions

M. Belli, V. Dini, G. Esposito, P. Micera, O. Sapora, C. Signoretti, G. Simone, B. Stenerlöw, M. A. Tabocchini

Research output: Contribution to journalArticle

Abstract

This study was aimed at investigating the radiation induced DNA fragmentation pattern as a function of cellular differentiation and radiation quality. DNA double strand breaks (DSB) induced by γ-rays were analyzed in K562 human proerithroblasts before (AP cells) and after (D cells) differentiation induction while DNA DSB induced by 125 keV/μm N-ions have been studied in AP cells. Pulsed-Field Gel Electrophoresis (PFGE) of cellular DNA was used to determine the DSB yield by analysis of the Fraction of Activity Released (FAR) and of the fragmentation pattern in a specific size range (5.7-0.225 Mbp). The results so far obtained show that the DSB induction by γ-rays is different if evaluated with the FAR or with the fragmentation analysis. The DSB yield obtained with the former method is about 1.4 times higher in AP respect to D cells while the latter method indicates that more fragments are produced in D cells. Comparison between γ-rays and N-ions in AP cells shows that no significant differences are detected by the FAR analysis; otherwise fragmentation analysis demonstrates a higher effectiveness of nitrogen ions.

Original languageEnglish
Pages (from-to)219-221
Number of pages3
JournalPhysica Medica
Volume17
Issue numberSUPPL. 1
Publication statusPublished - Sep 17 2001

Keywords

  • Cellular differentiation
  • DNA DSB
  • FAR analysis
  • Fragmentation analysis

ASJC Scopus subject areas

  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Physics and Astronomy(all)

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  • Cite this

    Belli, M., Dini, V., Esposito, G., Micera, P., Sapora, O., Signoretti, C., Simone, G., Stenerlöw, B., & Tabocchini, M. A. (2001). DNA fragmentation induced in K562 cells by nitrogen ions. Physica Medica, 17(SUPPL. 1), 219-221.