DNA fragmentation is a feature of cystic fibrosis epithelial cells: A disease with inappropriate apoptosis?

Luigi Maiuri, Valeria Raia, Giulio De Marco, Salvatore Coletta, Giorgio De Ritis, Marco Londei, Salvatore Auricchio

Research output: Contribution to journalArticlepeer-review

Abstract

Cystic fibrosis (CF) is a single-gene disease caused by mutations in the CFTR gene, which result in disrupted chloride secretions with inspissated mucous secretions by exocrine glands. Nick-end labelling was used to assess DNA fragmentation in 14 CF and 24 control duodenal samples, and in two CF and two control lung tissues. In CF small intestine median 46% (range: 30-82) villus enterocytes show DNA fragmentation (vs. 3% (range: 1-7) in controls P <0.001) and median 37.5% (range: 23-79) crypt enterocytes show Ki67 antigen (P <0.001). In CF airways 57% (range: 54-70) of epithelial cells show DNA fragmentation. Inappropriate high DNA fragmentation is a feature of various CF epithelia. This could have great impact in understanding the mechanisms leading to disease.

Original languageEnglish
Pages (from-to)225-231
Number of pages7
JournalFEBS Letters
Volume408
Issue number2
DOIs
Publication statusPublished - May 19 1997

Keywords

  • Apoptosis
  • Cystic fibrosis
  • Cystic fibrosis transmembrane conductance regulator
  • DNA fragmentation
  • Programmed cell death

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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