TY - JOUR
T1 - DNA fragmentation is a feature of cystic fibrosis epithelial cells
T2 - A disease with inappropriate apoptosis?
AU - Maiuri, Luigi
AU - Raia, Valeria
AU - De Marco, Giulio
AU - Coletta, Salvatore
AU - De Ritis, Giorgio
AU - Londei, Marco
AU - Auricchio, Salvatore
PY - 1997/5/19
Y1 - 1997/5/19
N2 - Cystic fibrosis (CF) is a single-gene disease caused by mutations in the CFTR gene, which result in disrupted chloride secretions with inspissated mucous secretions by exocrine glands. Nick-end labelling was used to assess DNA fragmentation in 14 CF and 24 control duodenal samples, and in two CF and two control lung tissues. In CF small intestine median 46% (range: 30-82) villus enterocytes show DNA fragmentation (vs. 3% (range: 1-7) in controls P <0.001) and median 37.5% (range: 23-79) crypt enterocytes show Ki67 antigen (P <0.001). In CF airways 57% (range: 54-70) of epithelial cells show DNA fragmentation. Inappropriate high DNA fragmentation is a feature of various CF epithelia. This could have great impact in understanding the mechanisms leading to disease.
AB - Cystic fibrosis (CF) is a single-gene disease caused by mutations in the CFTR gene, which result in disrupted chloride secretions with inspissated mucous secretions by exocrine glands. Nick-end labelling was used to assess DNA fragmentation in 14 CF and 24 control duodenal samples, and in two CF and two control lung tissues. In CF small intestine median 46% (range: 30-82) villus enterocytes show DNA fragmentation (vs. 3% (range: 1-7) in controls P <0.001) and median 37.5% (range: 23-79) crypt enterocytes show Ki67 antigen (P <0.001). In CF airways 57% (range: 54-70) of epithelial cells show DNA fragmentation. Inappropriate high DNA fragmentation is a feature of various CF epithelia. This could have great impact in understanding the mechanisms leading to disease.
KW - Apoptosis
KW - Cystic fibrosis
KW - Cystic fibrosis transmembrane conductance regulator
KW - DNA fragmentation
KW - Programmed cell death
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U2 - 10.1016/S0014-5793(97)00347-5
DO - 10.1016/S0014-5793(97)00347-5
M3 - Article
C2 - 9187372
AN - SCOPUS:0030926711
VL - 408
SP - 225
EP - 231
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2
ER -