Abstract
In cases of rape, obtaining enough biologic material for DNA identification of the attacker is often difficult because the methods for distinguishing and separating sperm cells from vaginal cells are not sufficiently efficacious. This article describes a new, innovative method for spermatic DNA extraction from the vaginal washing fluid by means of flow cytometry. The high specificity and sensitivity of the flow-cytometric sorting method provides enough sperm cells for DNA typing. The ease of execution of this method, involving vaginal washing with physiologic solution and flow-cytometric reading of the fresh sample, substantially increases its cost-benefit ratio.
| Original language | English |
|---|---|
| Pages (from-to) | 254-270 |
| Number of pages | 17 |
| Journal | American Journal of Forensic Medicine and Pathology |
| Volume | 24 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Sep 2003 |
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Keywords
- CODIS
- DNA typing
- Flow-cytometry
- Rape investigation
- STR systems
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Pathology and Forensic Medicine
- Law
Cite this
DNA identification of sperm cells collected and sorted by flow cytometry. / Di Nunno, Nunzio; Melato, Mauro; Vimercati, Antonella; Di Nunno, Cosimo; Costantinides, Fulvio; Vecchiotti, Carla; Frezzini, Cristina; Cina, Steven; Vimercati, Francesco.
In: American Journal of Forensic Medicine and Pathology, Vol. 24, No. 3, 09.2003, p. 254-270.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - DNA identification of sperm cells collected and sorted by flow cytometry
AU - Di Nunno, Nunzio
AU - Melato, Mauro
AU - Vimercati, Antonella
AU - Di Nunno, Cosimo
AU - Costantinides, Fulvio
AU - Vecchiotti, Carla
AU - Frezzini, Cristina
AU - Cina, Steven
AU - Vimercati, Francesco
PY - 2003/9
Y1 - 2003/9
N2 - In cases of rape, obtaining enough biologic material for DNA identification of the attacker is often difficult because the methods for distinguishing and separating sperm cells from vaginal cells are not sufficiently efficacious. This article describes a new, innovative method for spermatic DNA extraction from the vaginal washing fluid by means of flow cytometry. The high specificity and sensitivity of the flow-cytometric sorting method provides enough sperm cells for DNA typing. The ease of execution of this method, involving vaginal washing with physiologic solution and flow-cytometric reading of the fresh sample, substantially increases its cost-benefit ratio.
AB - In cases of rape, obtaining enough biologic material for DNA identification of the attacker is often difficult because the methods for distinguishing and separating sperm cells from vaginal cells are not sufficiently efficacious. This article describes a new, innovative method for spermatic DNA extraction from the vaginal washing fluid by means of flow cytometry. The high specificity and sensitivity of the flow-cytometric sorting method provides enough sperm cells for DNA typing. The ease of execution of this method, involving vaginal washing with physiologic solution and flow-cytometric reading of the fresh sample, substantially increases its cost-benefit ratio.
KW - CODIS
KW - DNA typing
KW - Flow-cytometry
KW - Rape investigation
KW - STR systems
UR - http://www.scopus.com/inward/record.url?scp=0041317063&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0041317063&partnerID=8YFLogxK
U2 - 10.1097/01.paf.0000070224.58005.ac
DO - 10.1097/01.paf.0000070224.58005.ac
M3 - Article
C2 - 12960662
AN - SCOPUS:0041317063
VL - 24
SP - 254
EP - 270
JO - American Journal of Forensic Medicine and Pathology
JF - American Journal of Forensic Medicine and Pathology
SN - 0195-7910
IS - 3
ER -