DNA injury is acutely enhanced in response to increasing bulks of aerobic physical exercise

Giuseppe Lippi, Ruggero Buonocore, Cantor Tarperi, Martina Montagnana, Luca Festa, Elisa Danese, Marco Benati, Gian Luca Salvagno, Chiara Bonaguri, Dirk Roggenbuck, Federico Schena

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The aim of this study was to evaluate DNA damage in response to increasing bulks of aerobic physical exercise. Fifteen adult and trained athletes performed four sequential trials with increasing running distance (5-, 10-, 21- and 42-km) in different periods of the year. The γ-H2AX foci parameters were analyzed before and 3 h after the end of each trial. The values of all γ-H2AX foci parameters were enhanced after the end of each trial, with values gradually increasing from the 5- to the 42-km trial. Interestingly, a minor increase of γ-H2AX foci was still evident after 5- to 10-km running, but a much higher increase occurred when the running distance exceeded 21 km. The generation of DNA injury was then magnified by running up to 42-km. The increase of each γ-H2AX foci parameter was then found to be associated with both running distance and average intensity. In multivariate linear regression analysis, the running distance was significantly associated with average intensity and post-run variation in the percentage of cells with γ-H2AX foci. We can hence conclude that aerobic exercise may generate an acute DNA damage in trained athletes, which is highly dependent upon running distance and average intensity.

Original languageEnglish
Pages (from-to)146-151
Number of pages6
JournalClinica Chimica Acta
Volume460
DOIs
Publication statusPublished - Sep 1 2016

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Running
DNA Damage
Exercise
DNA
Linear regression
Regression analysis
Athletes
Linear Models
Regression Analysis

Keywords

  • Aerobic physical exercise
  • DNA
  • Injury

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

DNA injury is acutely enhanced in response to increasing bulks of aerobic physical exercise. / Lippi, Giuseppe; Buonocore, Ruggero; Tarperi, Cantor; Montagnana, Martina; Festa, Luca; Danese, Elisa; Benati, Marco; Salvagno, Gian Luca; Bonaguri, Chiara; Roggenbuck, Dirk; Schena, Federico.

In: Clinica Chimica Acta, Vol. 460, 01.09.2016, p. 146-151.

Research output: Contribution to journalArticle

Lippi, G, Buonocore, R, Tarperi, C, Montagnana, M, Festa, L, Danese, E, Benati, M, Salvagno, GL, Bonaguri, C, Roggenbuck, D & Schena, F 2016, 'DNA injury is acutely enhanced in response to increasing bulks of aerobic physical exercise', Clinica Chimica Acta, vol. 460, pp. 146-151. https://doi.org/10.1016/j.cca.2016.06.041
Lippi, Giuseppe ; Buonocore, Ruggero ; Tarperi, Cantor ; Montagnana, Martina ; Festa, Luca ; Danese, Elisa ; Benati, Marco ; Salvagno, Gian Luca ; Bonaguri, Chiara ; Roggenbuck, Dirk ; Schena, Federico. / DNA injury is acutely enhanced in response to increasing bulks of aerobic physical exercise. In: Clinica Chimica Acta. 2016 ; Vol. 460. pp. 146-151.
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