DNA measurement by image analysis of paraffin-embedded breast carcinoma tissue: A comparative investigation

S. Bosari, B. D. Wiley, W. M. Hamilton, J. M. Dugan, L. B. Cook, A. K C Lee

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Measurement of cellular DNA content may provide useful prognostic information in several human neoplasms. The DNA content by image analysis of fresh tissue has been compared with flow cytometry with good correlation, but the use of paraffin-embedded tissue for image analysis has not been studied widely. This study reports the DNA content of 54 breast carcinomas and compares the results of image analysis of paraffin-embedded tissue and results of image analysis and flow cytometry of corresponding fresh tumors. Image analysis of paraffin blocks and fresh tumors showed comparable results in 51 tumors (94%), whereas 47 tumors (87%) were similar by all three methods. The DNA indices from image analysis of fresh and paraffin-embedded tumors showed significant correlation by linear regression analysis (r = 0.96; P <0.001). Discordances between image analysis of fresh and paraffin-embedded tumors are the result of technical problems, such as staining and tissue preservation. Discordances between image analysis and flow cytometry reflect the advantages and pitfalls of the two techniques. These data suggest that image analysis of paraffin-embedded tissue is a viable technique that will permit the performance of retrospective studies with long-term follow-up data for the evaluation of the prognostic significance of DNA content.

Original languageEnglish
Pages (from-to)698-703
Number of pages6
JournalAmerican Journal of Clinical Pathology
Volume96
Issue number6
Publication statusPublished - 1991

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Paraffin
Breast Neoplasms
DNA
Neoplasms
Image Cytometry
Flow Cytometry
Tissue Preservation
Linear Models
Retrospective Studies
Regression Analysis
Staining and Labeling

Keywords

  • Breast carcinoma
  • DNA measurement
  • Flow cytometry
  • Image analysis
  • Paraffin blocks

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Bosari, S., Wiley, B. D., Hamilton, W. M., Dugan, J. M., Cook, L. B., & Lee, A. K. C. (1991). DNA measurement by image analysis of paraffin-embedded breast carcinoma tissue: A comparative investigation. American Journal of Clinical Pathology, 96(6), 698-703.

DNA measurement by image analysis of paraffin-embedded breast carcinoma tissue : A comparative investigation. / Bosari, S.; Wiley, B. D.; Hamilton, W. M.; Dugan, J. M.; Cook, L. B.; Lee, A. K C.

In: American Journal of Clinical Pathology, Vol. 96, No. 6, 1991, p. 698-703.

Research output: Contribution to journalArticle

Bosari, S. ; Wiley, B. D. ; Hamilton, W. M. ; Dugan, J. M. ; Cook, L. B. ; Lee, A. K C. / DNA measurement by image analysis of paraffin-embedded breast carcinoma tissue : A comparative investigation. In: American Journal of Clinical Pathology. 1991 ; Vol. 96, No. 6. pp. 698-703.
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